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IL-12 p70 Antibody (500-P154GBT-25UG) in ELISA
Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL-12 p70 Polyclonal Antibody, Biotin (Product # 500-P154GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL-12 p70 Polyclonal Antibody (Product # 500-P154G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-12.
Product Details
500-P154GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
CHO cells-derived Recombinant Human IL-12 p70
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929519
Product Specific Information
AA Sequence of recombinant protein: p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS .
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-12. Anti-Human IL-12-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-12 (500-P154G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-12.
Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P154GBT-1MG will be provided as 2 x 500 µg
Target Information
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 20C2; CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL12, subunit p40; IL12A/B; IL12AB; IL35 subunit; ILN; Interleukin; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2
Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL12A; IL12B; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35
UniProt ID: (Human) P29459, (Human) P29460
Entrez Gene ID: (Human) 3592, (Human) 3593
cytokine activity
interleukin-12 receptor binding
protein binding
growth factor activity
interleukin-12 beta subunit binding
interleukin-27 binding
protein heterodimerization activity
cytokine receptor activity
interleukin-12 alpha subunit binding
identical protein binding
protein homodimerization activity
interleukin-23 receptor binding
transmembrane signal receptor
positive regulation of T cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
immune response
cell cycle arrest
response to virus
response to UV-B
cell migration
response to lipopolysaccharide
negative regulation of interleukin-17 production
positive regulation of interferon-gamma production
positive regulation of natural killer cell activation
positive regulation of mononuclear cell proliferation
positive regulation of smooth muscle cell apoptotic process
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat4 protein
defense response to protozoan
positive regulation of T cell differentiation
positive regulation of cell adhesion
positive regulation of natural killer cell mediated cytotoxicity
negative regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
defense response to Gram-positive bacterium
positive regulation of NK T cell activation
cellular response to lipopolysaccharide
extrinsic apoptotic signaling pathway
cellular response to virus
positive regulation of dendritic cell chemotaxis
positive regulation of defense response to virus by host
natural killer cell activation involved in immune response
positive regulation of T-helper 1 type immune response
negative regulation of inflammatory response to antigenic stimulus
positive regulation of activation of JAK2 kinase activity
cytokine-mediated signaling pathway
sensory perception of pain
sexual reproduction
natural killer cell activation
negative regulation of interleukin-10 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell proliferation
positive regulation of tissue remodeling
regulation of cytokine biosynthetic process
T-helper 1 type immune response
T-helper cell differentiation
interferon-gamma biosynthetic process
positive regulation of activated T cell proliferation
positive regulation of NF-kappaB import into nucleus
regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of memory T cell differentiation
negative regulation of growth of symbiont in host
positive regulation of interferon-gamma biosynthetic process
positive regulation of osteoclast differentiation
positive regulation of inflammatory response
defense response to Gram-negative bacterium
positive regulation of NK T cell proliferation
defense response to virus
cellular response to interferon-gamma
positive regulation of T-helper 17 type immune response
positive regulation of T-helper 17 cell lineage commitment
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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