Invitrogen

IL-12 p70 Monoclonal Antibody (9A5)

Product Details

MM120

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 7 publications 7 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Pig, Rat

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

9A5

Immunogen

Recombinant mouse IL-12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Caprylic acid precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223525

Product Specific Information

MM120 targets IL-12 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM120 immunogen is recombinant mouse IL-12.

MM120 detects IL-12 which has a predicted molecular weight of approximately 22 kDa.

The MM120 mouse IL12 antibody (clone 9A5) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM121B (biotinylated conjugate of clone C17-8). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM120 (clone 9A5) and biotinylated antibody MM121B (clone C17-8) have successfully been used in combination with recombinant mouse IL12p70 protein SMIL12 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes.

Infection and immunity

Dewamitta SR,Nomura T,Kawamura I,Hara H,Tsuchiya K,Kurenuma T,Shen Y,Daim S,Yamamoto T,Qu H,Sakai S,Xu Y,Mitsuyama M

MM120 was used in ELISA to investigate the role of listeriolysin O in the calpain activation and interleukin-1 alpha secretion in Listeria monocytogene-infected macrophages

Sat May 01 00:00:00 EDT 2010

Involvement of IL-10 in exhaustion of myeloid dendritic cells and rescue by CD40 stimulation.

Immunology

Kajino K,Nakamura I,Bamba H,Sawai T,Ogasawara K

MM120 was used in ELISA to study the role of interleukin 10 in the function of myeloid dendritic cells

Mon Jan 01 00:00:00 EST 2007

Protection of non-obese diabetic mice from autoimmune diabetes by Escherichia coli heat-labile enterotoxin B subunit.

Immunology

Ola TO,Williams NA

MM120 was used in immunohistochemistry to show that EtxB can block diabetes

Wed Feb 01 00:00:00 EST 2006

Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection.

Cancer research

Guiducci C,Vicari AP,Sangaletti S,Trinchieri G,Colombo MP

MM120 was used in ELISA to describe the mechanisms by which tumor-infiltrating macrophages and dendritic cells can be redirected to become potent effectors and activators of the innate and adaptive immunity

Fri Apr 15 00:00:00 EDT 2005

Interleukin 12 and antigen independently induce substance P receptor expression in T cells in murine schistosomiasis mansoni.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Blum AM,Metwali A,Crawford C,Li J,Qadir K,Elliott DE,Weinstock JV

MM120 was used in ELISA to investigate the promoting effect of interleukin 12 and schistosome egg antigen on substance P receptor expression

Sun Apr 01 00:00:00 EST 2001

Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.

Journal of immunology (Baltimore, Md. : 1950)

DeKruyff RH,Fang Y,Umetsu DT

MM120 was used in blocking/activating experiment to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells

Sun Mar 01 00:00:00 EST 1998

Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.

Journal of immunology (Baltimore, Md. : 1950)

Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH

MM120 was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses

Thu Oct 15 00:00:00 EDT 1998

Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.

European journal of immunology

Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G

MM120 was used in ELISA to investigate the role of interleukin 12 in Th1 cell function

Fri Mar 01 00:00:00 EST 1996

Characterization of early IL-12, IFN-alphabeta, and TNF effects on antiviral state and NK cell responses during murine cytomegalovirus infection.

Journal of immunology (Baltimore, Md. : 1950)

Orange JS,Biron CA

MM120 was used in ELISA to investigate the importance of tumor necrosis factor, interleukin 12 and interferon alpha beta in immune responses against cytomegalovirus infection

Sat Jun 15 00:00:00 EDT 1996

Hepatic Na(+)-K(+)-ATPase enzyme activity correlates with polarized beta-subunit expression.

The American journal of physiology

Simon FR,Leffert HL,Ellisman M,Iwahashi M,Deerinck T,Fortune J,Morales D,Dahl R,Sutherland E

MM120 was used in immunohistochemistry to investigate the relationship between hepatic sodium/potassium ATPase activity and the expression of polarized beta subunit

Sat Jul 01 00:00:00 EDT 1995

Identification of monoclonal antibody binding domains of Na+,K(+)-ATPase by immunoelectron microscopy.

FEBS letters

Ting-Beall HP,Beall HC,Hastings DF,Friedman ML,Ball WJ

MM120 was used in immunoprecipitation to investigate the binding domains of sodium/potassium ATPase antibody

Mon Jun 04 00:00:00 EDT 1990

Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping.

Biochemistry

Hubert JJ,Schenk DB,Skelly H,Leffert HL

MM120 was used in immunoprecipitation to investigate the structure of sodium/potassium ATPase in rat liver

Tue Jul 15 00:00:00 EDT 1986

Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver.

The Journal of biological chemistry

Schenk DB,Hubert JJ,Leffert HL

MM120 was used in blocking/activating experiment to investigate the activity and sites of sodium/potassium ATPase in normal and regenerating rat liver

Mon Dec 10 00:00:00 EST 1984

Bioinformatics

Protein Aliases: 20C2; CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL12A/B; IL12AB; ILN; Interleukin; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; Il12a; Il12b; Il12p40; Ll12a; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160

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Function(s)

transmembrane signal receptor

Performance Guarantee

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IL-12 p70 Monoclonal Antibody (9A5)

Product Details

MM120

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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