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Invitrogen
IL-13 Polyclonal Antibody, Biotin, eBioscience™
Product Details
13-7138-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_466933
Product Specific Information
Description: The polyclonal antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8^+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.
Applications Reported: The biotinylated anti-human IL-13 polyclonal antibody has been reported for use in ELISA and ELISPOT.
Applications Tested: The biotinylated anti-human IL-13 polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-13 in combination with the purified PVM13-1 (14-7139) antibody for capture and recombinant human IL-13 (14-8139) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant cytokine standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody
Gene Aliases: IL-13; IL13; NC30; P600
UniProt ID: (Human) P35225
Entrez Gene ID: (Human) 3596
microglial cell activation
positive regulation of immunoglobulin production
movement of cell or subcellular component
inflammatory response
immune response
signal transduction
cell-cell signaling
regulation of proton transport
positive regulation of B cell proliferation
response to lipopolysaccharide
positive regulation of connective tissue growth factor production
negative regulation of NAD(P)H oxidase activity
response to nicotine
positive regulation of tyrosine phosphorylation of Stat6 protein
positive regulation of macrophage activation
positive regulation of mast cell degranulation
response to ethanol
positive regulation of smooth muscle cell proliferation
positive regulation of protein secretion
positive regulation of release of sequestered calcium ion into cytosol
cellular response to mechanical stimulus
cellular response to cytokine stimulus
negative regulation of transforming growth factor beta production
negative regulation of neuron death
negative regulation of lung ciliated cell differentiation
positive regulation of lung goblet cell differentiation
negative regulation of complement-dependent cytotoxicity
positive regulation of pancreatic stellate cell proliferation
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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