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IL-13 Antibody (500-P224-1MG) in ELISA
Sandwich ELISA: To detect Rat IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-13 Polyclonal Antibody (Product # 500-P224-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-13 Polyclonal Antibody, Biotin (Product # 500-P224BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-13.
Product Details
500-P224-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat IL-13 (113 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929894
Product Specific Information
AA Sequence of recombinant protein: TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSMVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-13. Anti-Rat IL-13-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-13 matrix.
Sandwich ELISA: To detect Rat IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-13 (500-P224Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-13.
Western Blot: To detect Rat IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600
Gene Aliases: Il-13; Il13
UniProt ID: (Rat) P42203
Entrez Gene ID: (Rat) 116553
microglial cell activation
positive regulation of immunoglobulin production
inflammatory response
immune response
response to mechanical stimulus
regulation of proton transport
positive regulation of B cell proliferation
response to lipopolysaccharide
positive regulation of connective tissue growth factor production
negative regulation of NAD(P)H oxidase activity
response to nicotine
positive regulation of tyrosine phosphorylation of Stat6 protein
positive regulation of macrophage activation
positive regulation of ion transport
positive regulation of mast cell degranulation
response to ethanol
positive regulation of smooth muscle cell proliferation
positive regulation of protein secretion
positive regulation of release of sequestered calcium ion into cytosol
cellular response to mechanical stimulus
cellular response to cytokine stimulus
negative regulation of transforming growth factor beta production
negative regulation of neuron death
negative regulation of complement-dependent cytotoxicity
positive regulation of pancreatic stellate cell proliferation
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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