Invitrogen

IL-13 Polyclonal Antibody

Product Details

P130E

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human IL-13

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223471

Product Specific Information

P130E targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.

The P130E immunogen is recombinant human IL-13.

P130E detects IL-13 which has a predicted molecular weight of approximately 13 kDa.

The P130E IL13 polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M130B (biotinylated conjugate of clone 5A2) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody P130E and biotinylated antibody M130B (clone 5A2) have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IL-4/IL-13 remodeling pathway of COVID-19 lung injury.

Scientific reports

Vaz de Paula CB,de Azevedo MLV,Nagashima S,Martins APC,Malaquias MAS,Miggiolaro AFRDS,da Silva Motta Júnior J,Avelino G,do Carmo LAP,Carstens LB,de Noronha L

P130E was used in Immunohistochemistry (Paraffin) to conclude that understanding and managing the aggravated and ineffective immune response elicited by SARS-CoV-2 merits further clarification to improve treatments propose.

Thu Oct 29 00:00:00 EDT 2020

The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans.

Journal of immunology (Baltimore, Md. : 1950)

Lozano E,Joller N,Cao Y,Kuchroo VK,Hafler DA

P130E was used in ELISA to study the role of the interaction between CD226 and CD155 in regulating the human T-cell pro-inflammatory/anti-inflammatory balance

Tue Oct 01 00:00:00 EDT 2013

Fibroblasts stimulated via HLA-II molecules produce prostaglandin E₂ and regulate cytokine production from helper T cells.

Laboratory investigation; a journal of technical methods and pathology

Kato-Kogoe N,Ohyama H,Nishimura F,Meguro M,Yoshizawa S,Okada Y,Nakasho K,Yamanegi K,Yamada N,Hata M,Higashi T,Terada N,Matsushita S

P130E was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses

Wed Dec 01 00:00:00 EST 2010

Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya.

PLoS medicine

Malhotra I,Dent A,Mungai P,Wamachi A,Ouma JH,Narum DL,Muchiri E,Tisch DJ,King CL

P130E was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance

Wed Jul 01 00:00:00 EDT 2009

Modulation of the infant immune responses by the first pertussis vaccine administrations.

Vaccine

Mascart F,Hainaut M,Peltier A,Verscheure V,Levy J,Locht C

P130E was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines

Thu Jan 04 00:00:00 EST 2007

Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands.

Infection and immunity

Malhotra I,Mungai P,Muchiri E,Ouma J,Sharma S,Kazura JW,King CL

P130E was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands

Wed Jun 01 00:00:00 EDT 2005

Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans.

Journal of immunology (Baltimore, Md. : 1950)

Xainli J,Baisor M,Kastens W,Bockarie M,Adams JH,King CL

P130E was used in ELISA to investigate the role of plasmodium vivax duffy binding protein

Sun Sep 15 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody

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Gene Aliases: IL-13; IL13; NC30; P600

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UniProt ID: (Human) P35225

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Entrez Gene ID: (Human) 3596

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Function(s)

cytokine activity interleukin-13 receptor binding protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-13 Polyclonal Antibody

Product Details

P130E

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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