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IL-16 Antibody (500-P06-1MG) in WB
Western Blot: To detect Human IL-16 by Western Blot analysis IL-16 Polyclonal Antibody (Product # 500-P06-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P06-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-16 (129 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929166
Product Specific Information
AA Sequence of recombinant protein: PDLNSSTDSA ASASAASDVS VESTAEATVC TVTLEKMSAG LGFSLEGGKG SLHGDKPLTI NRIFKGAASE QSETVQPGDE ILQLGGTAMQ GLTRFEAWNI IKALPDGPVT IVIRRKSLQS KETTAAGDS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-16. Anti-Human IL-16-specific antibody was purified by affinity chromatography employing an immobilized Human IL-16 matrix.
Sandwich ELISA: To detect Human IL-16 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-16 (500-P06Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-16.
Western Blot: To detect Human IL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of IL-16 undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ16806; FLJ42735; FLJ44234; H-IL-16; il 16; ILN; Interleukin; Interleukin16; lymphocyte chemoattractant factor; M-IL-16; neuronal interleukin 16; Pro-interleukin-16; prointerleukin 16
Gene Aliases: IL16; LCF; NIL16; prIL-16; PRIL16
UniProt ID: (Human) Q14005
Entrez Gene ID: (Human) 3603
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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