Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
IL-17 A/F Monoclonal Antibody (50104)
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
IL-17 A/F Antibody (MA5-23748) in IHC
PLoS pathogens 2022 -
10.1371/journal.ppat.1010185.g004 Fig 4 IL-17-expressing cells infiltrate joint tissues during RRV disease. A) Confocal microscopy of immunofluorescent staining of optically cleared 150mum-thick vibratome sections of feet from mock-infected or RRV-infected IL-17 GFP mice, at 10 dpi. Scale bars = 50mum. Wholemount sections were stained for Type IV Collagen (red) and nuclei labelled with DAPI (blue). B) Immun... View More
Product Details
MA5-23748
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
50104
Immunogen
E. coli-derived recombinant mouse IL-17 Thr22-Ala158
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607352
Product Specific Information
In direct ELISAs, approximately 40% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant human IL-17, recombinant canine IL-17, rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; IL-17F; ILN; Interleukin; Interleukin-17A; Interleukin-17F
Gene Aliases: C87042; Ctla-8; Ctla8; IL-17; IL-17A; IL-17F; Il17; Il17a; Il17f
UniProt ID: (Mouse) Q62386, (Mouse) Q7TNI7
Entrez Gene ID: (Mouse) 16171, (Mouse) 257630
inflammatory response
positive regulation of necrotic cell death
positive regulation of interleukin-23 production
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to interleukin-1
cellular response to glucocorticoid stimulus
fibroblast activation
positive regulation of cytokine production involved in inflammatory response
proteoglycan metabolic process
negative regulation of angiogenesis
regulation of transforming growth factor beta receptor signaling pathway
cytokine biosynthetic process
lymphotoxin A biosynthetic process
regulation of interleukin-2 biosynthetic process
regulation of interleukin-6 biosynthetic process
regulation of interleukin-8 biosynthetic process
regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
cartilage development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support