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IL-17A Antibody (500-P07GBT-25UG) in ELISA
Sandwich ELISA: To detect Human IL-17A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL-17A Polyclonal Antibody, Biotin (Product # 500-P07GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL-17A Polyclonal Antibody (Product # 500-P07G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17A.
Product Details
500-P07GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-17A
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929181
Product Specific Information
AA Sequence of recombinant protein: MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-17A. Anti-Human IL-17A-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IL-17A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-17A (500-P07G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17A.
Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P07GBT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A
Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A
UniProt ID: (Human) Q16552
Entrez Gene ID: (Human) 3605
apoptotic process
inflammatory response
immune response
cell surface receptor signaling pathway
cell-cell signaling
cell death
positive regulation of necrotic cell death
positive regulation of interleukin-23 production
response to amino acid
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
defense response to fungus
cellular response to interleukin-1
cellular response to glucocorticoid stimulus
fibroblast activation
positive regulation of cytokine production involved in inflammatory response
positive regulation of interleukin-6 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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