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IL-17f Antibody (MA5-24038) in Flow
Flow cytometry of IL-17f in Mouse splenocytes were treated for 24 hr with 50 ng/mL PMA, 200 ng/mL Ca 2+ ionomycin, 10 ng/mL Recombinant Human TGF‚β , and 40 ng/mL Recombinant Mouse IL‚6. Samples were incubated in IL-17f monoclonal antibody (Product # MA5-24038) or isotype control antibody followed by Phycoerythrin‚conjugated Anti-Rat IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Product Details
MA5-24038
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2A
Class
Monoclonal
Type
Antibody
Clone
316016
Immunogen
E. coli-derived recombinant mouse IL-17F Arg29-Ala161
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607086
Product Specific Information
In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-17, rmIL-17B, C, D, E, or recombinant human IL-17F is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflammation by increasing chemokine production. IL-17F also stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17F is produced, and exists, as a homo-dimer, with homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells. IL-17F has been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IL-17F; ILN; Interleukin; Interleukin-17F; interleukin-24; Interleukin17F; mutant IL-17F
Gene Aliases: C87042; IL-17F; Il17f
UniProt ID: (Mouse) Q7TNI7
Entrez Gene ID: (Mouse) 257630
proteoglycan metabolic process
inflammatory response
negative regulation of angiogenesis
regulation of transforming growth factor beta receptor signaling pathway
cytokine biosynthetic process
lymphotoxin A biosynthetic process
regulation of interleukin-2 biosynthetic process
regulation of interleukin-6 biosynthetic process
regulation of interleukin-8 biosynthetic process
regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
cartilage development
positive regulation of cytokine production involved in inflammatory response
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