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Invitrogen
IL-18 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
IL-18 Antibody (PA5-47803) in Neu
Neutralization of IL-18 in KG‚1 human acute myelogenous leukemia cell line. Samples were incubated in IL-18 polyclonal antibody (Product # PA5-47803). In the presence of Recombinant Human TNF‚α , Recombinant Feline IL‚18/IL‚1F4 stimulates IFN‚γ secretion in the KG‚1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN‚γ Quantikine ELISA Kit. Under these conditions, IFN‚γ secretion elicited by Recombinant Feline IL‚18/IL‚1F4 (1 µg/mL) is neutralized (green line) by increasing conc... View More
Product Details
PA5-47803
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat
Published species
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant feline IL-18/IL-1F4 Tyr36-Asn192
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606212
Product Specific Information
In direct ELISAs, approximately 50% cross-reactivity with recombinant canine IL-18, approximately 30% cross-reactivity with recombinant porcine IL-18, approximately 20% cross-reactivity with recombinant human IL-18 and recombinant rat IL-18, and less than 10% cross-reactivity with recombinant mouse IL-18 and recombinant rhesus macaque IL-18 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; interferon gamma inducing factor; Interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
Gene Aliases: IGIF; IL18
UniProt ID: (Cat) Q95M33
Entrez Gene ID: (Cat) 493688
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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