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IL-18 Antibody (PA5-79482) in IHC (P)
Immunohistochemical analysis of IL18 in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-IL18 antibody (Product # PA5-79482) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary ... View More
Product Details
PA5-79482
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived rat IL-18 recombinant protein (Position: H37-S194).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2746598
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
Gene Aliases: Igif; IL-18; Il18
UniProt ID: (Mouse) P70380, (Rat) P97636
Entrez Gene ID: (Mouse) 16173, (Rat) 29197
MAPK cascade
angiogenesis
response to hypoxia
inflammatory response
immune response
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of gene expression
positive regulation of smooth muscle cell migration
natural killer cell activation
regulation of cell adhesion
sleep
lipopolysaccharide-mediated signaling pathway
positive regulation of chemokine production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell proliferation
positive regulation of superoxide anion generation
positive regulation of collagen biosynthetic process
negative regulation of neutrophil apoptotic process
T-helper 1 type immune response
interferon-gamma biosynthetic process
positive regulation of activated T cell proliferation
interleukin-13 biosynthetic process
positive regulation of NF-kappaB import into nucleus
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of apoptotic process
positive regulation of vascular permeability
positive regulation of neuron apoptotic process
negative regulation of myoblast differentiation
positive regulation of smooth muscle cell proliferation
negative regulation of peptidyl-tyrosine phosphorylation
detection of mechanical stimulus involved in sensory perception of pain
positive regulation of NK T cell proliferation
cellular response to lipopolysaccharide
cellular response to peptide hormone stimulus
cellular response to organic cyclic compound
positive regulation of male germ cell proliferation
positive regulation of blood vessel remodeling
osteoblast differentiation
learning or memory
response to radiation
response to cold
lung development
positive regulation of interleukin-1 beta production
response to peptide hormone
regulation of circadian sleep/wake cycle, non-REM sleep
digestive tract morphogenesis
response to hyperoxia
cellular response to hydrogen peroxide
cellular response to mechanical stimulus
cellular response to cAMP
cellular response to interferon-gamma
cellular response to tumor necrosis factor
cellular response to parathyroid hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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