Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
IL-18 Antibody (MA5-43740) in WB
Western blot analysis of IL-18 in Lane 1: HepG2 cell lysate; Lane 2: HeLa cell lysate; Lane 3: porcine Spleen lysate; Lane 4: rat Spleen lysate. Samples were incubated in monoclonal IL-18 antibody (Product # MA5-43740) using a dilution of 2 µg/mL, followed by HRP-Linked Caprine Anti-Mouse IgG at a dilution of 0.2 µg/mL.
Product Details
MA5-43740
Product Specifications
Species Reactivity
Human,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
C10
Immunogen
Arg94-Asp193 (Accession Q14116), with N-terminal His Tag
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2912672
Target Information
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cytokine; Iboctadekin; IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
Gene Aliases: IGIF; IL-18; IL-1g; IL18; IL1F4
UniProt ID: (Human) Q14116, (Pig) O19073, (Rat) P97636
Entrez Gene ID: (Human) 3606, (Pig) 397057, (Rat) 29197
MAPK cascade
angiogenesis
inflammatory response
immune response
cell-cell signaling
positive regulation of gene expression
natural killer cell activation
regulation of cell adhesion
sleep
lipopolysaccharide-mediated signaling pathway
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of natural killer cell proliferation
positive regulation of tissue remodeling
chemokine biosynthetic process
T-helper 1 type immune response
type 2 immune response
interleukin-2 biosynthetic process
interferon-gamma biosynthetic process
positive regulation of activated T cell proliferation
interleukin-13 biosynthetic process
granulocyte macrophage colony-stimulating factor biosynthetic process
positive regulation of NF-kappaB import into nucleus
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of myoblast differentiation
positive regulation of inflammatory response
positive regulation of NK T cell proliferation
cellular response to organic cyclic compound
cell proliferation
positive regulation of cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of macrophage derived foam cell differentiation
positive regulation of phosphatidylinositol 3-kinase signaling
activation of protein kinase B activity
interferon-gamma production
interleukin-6 production
interleukin-18-mediated signaling pathway
T-helper 1 cell cytokine production
neutrophil activation
natural killer cell mediated cytotoxicity
positive regulation of tyrosine phosphorylation of STAT protein
cholesterol homeostasis
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling
triglyceride homeostasis
positive regulation of cold-induced thermogenesis
positive regulation of NIK/NF-kappaB signaling
positive regulation of T-helper 1 cell cytokine production
osteoblast differentiation
response to hypoxia
learning or memory
response to radiation
response to cold
positive regulation of smooth muscle cell migration
lung development
positive regulation of chemokine production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of superoxide anion generation
positive regulation of collagen biosynthetic process
negative regulation of neutrophil apoptotic process
positive regulation of apoptotic process
positive regulation of vascular permeability
response to peptide hormone
positive regulation of neuron apoptotic process
regulation of circadian sleep/wake cycle, non-REM sleep
digestive tract morphogenesis
negative regulation of peptidyl-tyrosine phosphorylation
detection of mechanical stimulus involved in sensory perception of pain
response to hyperoxia
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular response to cAMP
cellular response to interferon-gamma
cellular response to tumor necrosis factor
cellular response to parathyroid hormone stimulus
cellular response to peptide hormone stimulus
positive regulation of male germ cell proliferation
positive regulation of blood vessel remodeling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support