Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
IL-18 Chimeric Recombinant Rabbit Monoclonal Antibody (125-2H)
FIGURE: 1 / 1
IL-18 Chimeric Antibody (MA5-47795) in WB
Western Blot analysis of IL-18 was performed using IL-18 Chimeric Monoclonal Antibody (Product # MA5-47795). HeLa cell lysate samples (35 µg protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with IL-18 Chimeric Monoclonal Antibody at 2 µg/mL before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3 kDa. IL-18 Chimeric Monoclonal Antibody successfully detected IL18 in HeLa cell lysate samples.
Product Details
MA5-47795
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
125-2H
Immunogen
Fusion of splenocytes from mice immunized with human IL-18, with SP2/O mouse myeloma cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074243
Product Specific Information
Specificity: Recognizes the active form of human IL-18.
Target Information
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Iboctadekin; IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; Interleukin; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
Gene Aliases: IGIF; IL-18; IL-1g; IL18; IL1F4
UniProt ID: (Human) Q14116
Entrez Gene ID: (Human) 3606
MAPK cascade
angiogenesis
inflammatory response
immune response
cell-cell signaling
positive regulation of gene expression
natural killer cell activation
regulation of cell adhesion
sleep
lipopolysaccharide-mediated signaling pathway
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of natural killer cell proliferation
positive regulation of tissue remodeling
chemokine biosynthetic process
T-helper 1 type immune response
type 2 immune response
interleukin-2 biosynthetic process
interferon-gamma biosynthetic process
positive regulation of activated T cell proliferation
interleukin-13 biosynthetic process
granulocyte macrophage colony-stimulating factor biosynthetic process
positive regulation of NF-kappaB import into nucleus
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of myoblast differentiation
positive regulation of inflammatory response
positive regulation of NK T cell proliferation
cellular response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support