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IL-2 Antibody (500-M127-500UG) in ELISA
Sandwich ELISA: Assuming 100 µL/well, a concentration of 4.0-8.0 µg/mL of IL-2 Monoclonal Antibody (Product # 500-M127-500UG) will detect at least 100 pg/mL of Recombinant Murine IL-2 when used with PeproTech IL-2 Polyclonal Antibody, Biotin (Product # 500-P111BT-1MG) as the detection antibody at a concentration of approximately 1.0- 2.0 µg/mL.
Product Details
500-M127-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
Class
Monoclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-2
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_3074160
Product Specific Information
AA Sequence of recombinant protein: PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ.
Preparation: Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-2. Anti-Murine IL-2-specific antibody was purified from cell culture by Protein A affinity chromatography.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: Il-2; Il2
UniProt ID: (Mouse) P04351
Entrez Gene ID: (Mouse) 16183
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
immune system process
negative regulation of B cell apoptotic process
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
protein kinase C-activating G-protein coupled receptor signaling pathway
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
negative regulation of heart contraction
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of immunoglobulin secretion
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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