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Invitrogen
IL-2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
IL-2 Antibody (PA5-47850) in ICC/IF
Immunocytochemistry analysis of IL-2 in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA. Samples were incubated in IL-2 polyclonal antibody (Product # PA5-47850) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47850
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant porcine IL-2 Ala21-Thr154
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609096
Product Specific Information
In sandwich ELISAs, approximately 3% cross-reactivity with recombinant canine IL-2 is observed, approximately 1% cross reactivity with recombinant human IL-2 and recombinant porcine IL-2 is observed, and less than 0.2% cross-reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: IL-2; IL2; POIL2; TCGF
UniProt ID: (Pig) P26891
Entrez Gene ID: (Pig) 396868
adaptive immune response
leukocyte activation involved in immune response
negative regulation of B cell apoptotic process
gene expression
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of immunoglobulin secretion
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of T-helper 17 cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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