Invitrogen

IL-2 Monoclonal Antibody (5H4)

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IL-2 Antibody (MM101) in ELISA

Sandwich ELISA analysis of mouse IL-2 was performed using the a Mouse IL-2 Colorimetric ELISA Kit (Product # EMIL2) by loading 50 µL per well of an anti-mouse IL-2 monoclonal antibody (Product # MM100) in quadruplicate at 0 (blue line), 50% (2.5 µg/mL, red line), and 100% (5 µg/mL, green line) displacement in a plate and then loading 50 µL per well of Mouse IL-2 Recombinant Protein (Product # SMIL2) in dodecuplicate at 850, 170, 34, and 0 pg/mL across a 5 µg/mL Mouse IL-2 (Product # MM101) pre-coated plate and incubating for 2 hours at 37°... View More

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Product Details

MM101

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 11 publications 11 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5H4

Immunogen

Recombinant mouse IL-2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223524

Product Specific Information

MM101 targets IL-2 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM101 immunogen is recombinant mouse IL-2.

MM101 detects IL-2 which has a predicted molecular weight of approximately 17 kDa.

The MM101 mouse IL2 antibody (clone 5H4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM102B (biotinylated conjugate of clone 1A12). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM101 (clone 5H4) and biotinylated antibody MM102B (clone 1A12) have successfully been used in combination with recombinant mouse IL2 protein SMIL2 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An atypical CD8 T-cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin-13.

Immunology

Johnson RM,Kerr MS,Slaven JE

MM101 was used in ELISA to study the mechanisms underlying CD8 T-cell-mediated pathology during genital tract Chlamydia infections and the significance of IL-13-producing cells

Sun Jun 01 00:00:00 EDT 2014

Plac8-dependent and inducible NO synthase-dependent mechanisms clear Chlamydia muridarum infections from the genital tract.

Journal of immunology (Baltimore, Md. : 1950)

Johnson RM,Kerr MS,Slaven JE

MM101 was used in ELISA to investigate Plac8-dependent and iNOS-dependent mechanisms of clearing Chlamydia muridarum genital tract infections

Wed Feb 15 00:00:00 EST 2012

PmpG303-311, a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice.

Infection and immunity

Johnson RM,Yu H,Kerr MS,Slaven JE,Karunakaran KP,Brunham RC

MM101 was used in ELISA to study a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice

Fri Jun 01 00:00:00 EDT 2012

Toxoplasma gondii glycosylphosphatidylinositols up-regulate major histocompatibility complex (MHC) molecule expression on primary murine macrophages.

Innate immunity

Debierre-Grockiego F,Molitor N,Schwarz RT,Lüder CG

MM101 was used in ELISA to study the effect of T. gondii glycosylphosphatidylinositols on MHC expression in macrophages

Sun Feb 01 00:00:00 EST 2009

Up-regulation of IL-4 production by the activated cAMP/cAMP-dependent protein kinase (protein kinase A) pathway in CD3/CD28-stimulated naive T cells.

International immunology

Tokoyoda K,Tsujikawa K,Matsushita H,Ono Y,Hayashi T,Harada Y,Abe R,Kubo M,Yamamoto H

MM101 was used in ELISA to investigate the role of cAMP/PKA pathway for the modulation of interleukin 4 expression in T cells

Sat May 01 00:00:00 EDT 2004

Murine strain differences in airway inflammation caused by diesel exhaust particles.

The European respiratory journal

Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M

MM101 was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants

Sun Feb 01 00:00:00 EST 1998

Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.

Journal of immunology (Baltimore, Md. : 1950)

DeKruyff RH,Fang Y,Umetsu DT

MM101 was used in blocking/activating experiment to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells

Sun Mar 01 00:00:00 EST 1998

Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A

MM101 was used in ELISA to study the mechanism for T cell differentiation

Tue Sep 15 00:00:00 EDT 1998

Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.

The Journal of pharmacology and experimental therapeutics

Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M

MM101 was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression

Sat Aug 01 00:00:00 EDT 1998

Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.

Toxicological sciences : an official journal of the Society of Toxicology

Ichinose T,Takano H,Miyabara Y,Sagai M

MM101 was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system

Wed Jul 01 00:00:00 EDT 1998

Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.

American journal of respiratory and critical care medicine

Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M

MM101 was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression

Tue Jul 01 00:00:00 EDT 1997

Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.

Journal of immunology (Baltimore, Md. : 1950)

Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E

MM101 was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation

Mon Jul 15 00:00:00 EDT 1996

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: Il-2; Il2

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UniProt ID: (Mouse) P04351

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Entrez Gene ID: (Mouse) 16183

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Function(s)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (5H4)

IL-2 Antibody (MM101) in ELISA

Product Details

MM101

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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