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IL-20 Antibody (500-P190GBT-25UG) in WB
Western Blot: To detect Human IL-20 by Western Blot analysis IL-20 Polyclonal Antibody, Biotin (Product # 500-P190GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P190GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-20
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929745
Product Specific Information
AA Sequence of recombinant protein: MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-20. Anti-Human IL-20-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIL-20 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-20 (500-P190G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-20.
Western Blot: To detect Human IL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P190GBT-1MG will be provided as 2 x 500 µg
Target Information
Cytokines that signal through Class II receptors form a distinct family that includes the interferons and interleukin 10 (IL-10). Recent identification of several IL-10 homologs has defined a cytokine subfamily that includes AK155, IL-19, IL-20, IL-22, and IL-24. IL-20 belongs to the IL-10 family and plays a role in skin inflammation and the development of hematopoietic cells. Little is known about its other biological functions and clinical implications. These cytokines display pro-inflammatory activities in different tissues, including skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine Zcyto10; four alpha helix cytokine; il 20; IL-20; ILN; Interleukin; Interleukin-20; MGC96907; PRO1801; UNQ852
Gene Aliases: IL-20; IL10D; IL20; UNQ852/PRO1801; ZCYTO10
UniProt ID: (Human) Q9NYY1
Entrez Gene ID: (Human) 50604
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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