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IL-21 Antibody (500-P278BT-25UG) in ELISA
Sandwich ELISA: To detect mIL-21 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL-21 Polyclonal Antibody, Biotin (Product # 500-P278BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL-21 Polyclonal Antibody (Product # 500-P278-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-21.
Product Details
500-P278BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-21
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930216
Product Specific Information
AA Sequence of recombinant protein: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-21. Anti-Murine IL-21-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mIL-21 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-21 (500-P278) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-21.
Western Blot: To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-21 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P278BT-1MG will be provided as 2 x 500 µg
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CX2; il 21; IL-21; ILN; Interleukin; Interleukin-21; Interleukin21
Gene Aliases: IL-21; Il21
UniProt ID: (Mouse) Q9ES17
Entrez Gene ID: (Mouse) 60505
B cell apoptotic process
positive regulation of cytokine production
positive regulation of natural killer cell cytokine production
immune response
positive regulation of cell proliferation
positive regulation of B cell proliferation
positive regulation of interleukin-10 production
positive regulation of interleukin-17 production
positive regulation of natural killer cell differentiation
positive regulation of T cell proliferation
tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of interferon-gamma biosynthetic process
positive regulation of B cell differentiation
positive regulation of natural killer cell mediated cytotoxicity
cell maturation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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