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Invitrogen
IL-21 Monoclonal Antibody (FFA21), APC, eBioscience™
FIGURE: 1 / 4
IL-21 Antibody (17-7211-82) in Flow
Intracellular staining of Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.125 µg of Anti-Mouse IL-21 APC. Cells were polarized for 9 days and then treated for 5 hours with Brefeldin A alone (Product # 00-4506-51) (left) or PMA/Ionomycin and Brefeldin A (right). Cells in the lymphocyte gate were used for analysis.
Product Details
17-7211-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
FFA21
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2016709
Product Specific Information
Description: This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21). IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1, IL-4 for Th2 and IL-17A for Th17, IL-21 is critical for TFH cell effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells.
In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules.
Preliminary data suggest that clone FFA21 recognizes a different epitope than anti-mouse IL-21 clone mhalx21 (Product # 51-7213-82).
Applications Reported: This FFA21 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This FFA21 antibody has been tested by intracellular staining and flow cytometric analysis on Th17-polarized splenocytes restimulated with PMA, Ionomycin and Brefeldin A for 5 hours. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CX2; il 21; IL-21; ILN; Interleukin; Interleukin-21; Interleukin21
Gene Aliases: IL-21; Il21
UniProt ID: (Mouse) Q9ES17
Entrez Gene ID: (Mouse) 60505
B cell apoptotic process
positive regulation of cytokine production
positive regulation of natural killer cell cytokine production
immune response
positive regulation of cell proliferation
positive regulation of B cell proliferation
positive regulation of interleukin-10 production
positive regulation of interleukin-17 production
positive regulation of natural killer cell differentiation
positive regulation of T cell proliferation
tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of interferon-gamma biosynthetic process
positive regulation of B cell differentiation
positive regulation of natural killer cell mediated cytotoxicity
cell maturation
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