Invitrogen

IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), PE, eBioscience™

FIGURE: 1 / 7

IL-21 Antibody (12-7219-42) in Flow

Intracellular staining of normal human peripheral blood cells treated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-42) (left) or Anti-Human IL-21 PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-7219-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio3A3-N2 (3A3-N2)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582260

Product Specific Information

Description: The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.

Applications Reported: This eBio3A3-N2 (3A3-N2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio3A3-N2 (3A3-N2) antibody has been pre-titrated and tested by intracellular staining of restimulated, Th17-polarized normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining for IL-21 has shown donor variability. stimulation times may vary depending on the donor and should be optimized. Increased staining levels have been observed on Th17-polarized normal human peripheral blood cells.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.

Cell

Rosain J,Neehus AL,Manry J,Yang R,Le Pen J,Daher W,Liu Z,Chan YH,Tahuil N,Türel Ö,Bourgey M,Ogishi M,Doisne JM,Izquierdo HM,Shirasaki T,Le Voyer T,Guérin A,Bastard P,Moncada-Vélez M,Han JE,Khan T,Rapaport F,Hong SH,Cheung A,Haake K,Mindt BC,Pérez L,Philippot Q,Lee D,Zhang P,Rinchai D,Al Ali F,Ahmad Ata MM,Rahman M,Peel JN,Heissel S,Molina H,Kendir-Demirkol Y,Bailey R,Zhao S,Bohlen J,Mancini M,Seeleuthner Y,Roelens M,Lorenzo L,Soudée C,Paz MEJ,González ML,Jeljeli M,Soulier J,Romana S,L'Honneur AS,Materna M,Martínez-Barricarte R,Pochon M,Oleaga-Quintas C,Michev A,Migaud M,Lévy R,Alyanakian MA,Rozenberg F,Croft CA,Vogt G,Emile JF,Kremer L,Ma CS,Fritz JH,Lemon SM,Spaan AN,Manel N,Abel L,MacDonald MR,Boisson-Dupuis S,Marr N,Tangye SG,Di Santo JP,Zhang Q,Zhang SY,Rice CM,Béziat V,Lachmann N,Langlais D,Casanova JL,Gros P,Bustamante J

Published figure using IL-21 monoclonal antibody (Product # 12-7219-42) in Flow Cytometry

Thu Feb 02 00:00:00 EST 2023

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

12-7219 was used in Flow cytometry/Cell sorting to perform a longitudinal assessment of individuals recovered from mild COVID-19 to determine whether they develop and sustain multifaceted SARS-CoV-2-specific immunological memory.

Thu Jan 07 00:00:00 EST 2021

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

12-7219-42 was used in Flow Cytometry to show increased formation of Tfh precursors (pre-Tfh) but no associated increase in germinal centre (GC)-Tfh cells in aged mice, suggesting age-driven promotion of only early Tfh cell differentiation.

Fri Jan 01 00:00:00 EST 2021

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

Fri Jan 01 00:00:00 EST 2021

A delayed fractionated dose RTS,S AS01 vaccine regimen mediates protection via improved T follicular helper and B cell responses.

eLife

Pallikkuth S,Chaudhury S,Lu P,Pan L,Jongert E,Wille-Reece U,Pahwa S

12-7219 was used in Flow cytometry/Cell sorting to conclude that the DFD regimen favors persistence of functional B cells after the third dose.

Wed Apr 29 00:00:00 EDT 2020

Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection.

Hepatology (Baltimore, Md.)

Cobb DA,Kim OK,Golden-Mason L,Rosen HR,Hahn YS

12-7219 was used in Flow cytometry/Cell sorting to examine the accumulation of Tfr cells in the liver compartment during chronic HCV infection and defined the cellular and molecular mechanisms underlying their expansion.

Mon Jan 01 00:00:00 EST 2018

Effects of Anti-TNFα Treatment on Mucosal Expression of IL-17A, IL-21, and IL-22 and Cytokine-Producing T Cell Subsets in Crohn's Disease.

Mediators of inflammation

Dige A,Magnusson MK,Uhrenholt C,Rasmussen TK,Kragstrup T,Öhman L,Dahlerup J,Agnholt J

12-7219 was used in Flow cytometry/Cell sorting to show that anti-TNFα treatment has no effect on mucosal IL-17A, IL-21, and IL-22 levels and cytokine-producing T cells in Crohn's diesease.

Mon Oct 01 00:00:00 EDT 2018

C5aR1 regulates T follicular helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans.

JCI insight

Verghese DA,Chun N,Paz K,Fribourg M,Woodruff TM,Flynn R,Hu Y,Xiong H,Zhang W,Yi Z,Du J,Blazar BR,Heeger PS

Published figure using IL-21 monoclonal antibody (Product # 12-7219-42) in Flow Cytometry

Thu Dec 20 00:00:00 EST 2018

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

12-7219-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

Activated T follicular helper-like cells are released into blood after oral vaccination and correlate with vaccine specific mucosal B-cell memory.

Scientific reports

Cárdeno A,Magnusson MK,Quiding-Järbrink M,Lundgren A

12-7219 was used in Flow cytometry/Cell sorting to investigate the frequencies, phenotype and function of circulating Tfh-like CD4+CXCR5+T cells in adults receiving an oral inactivated enterotoxigenic Escherichia coli vaccine.

Fri Feb 09 00:00:00 EST 2018

Th1 is the predominant helper T cell subset that produces GM-CSF in the joint of rheumatoid arthritis.

RMD open

Yamada H,Haraguchi A,Sakuraba K,Okazaki K,Fukushi JI,Mizu-Uchi H,Akasaki Y,Esaki Y,Kamura S,Fujimura K,Kondo M,Miyahara H,Nakashima Y,Yoshikai Y

12-7219 was used in Flow cytometry/Cell sorting to suggest the presence of common developmental pathway for these T cells.

Wed Nov 20 00:00:00 EST 2019

Cytotoxic T Cell Functions Accumulate When CD4 Is Downregulated by CD4⁺ T Cells in African Green Monkeys.

Journal of immunology (Baltimore, Md. : 1950)

Vinton CL,Ortiz AM,Calantone N,Mudd JC,Deleage C,Morcock DR,Whitted S,Estes JD,Hirsch VM,Brenchley JM

12-7219 was used in Flow cytometry/Cell sorting to study the functional profiles and SIV infection status in vivo of CD4+ T cells, CD8αα T cells, and CD8αΒ T cells of African green monkeys and rhesus macaques.

Thu Jun 01 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

12-7219 was used in Flow cytometry/Cell sorting to reveal that TIGIT+CD4+T cells provide a supportive microenvironment for CLL cells.

Sat Jan 29 00:00:00 EST 2022

Overexpression of microRNA-155 increases IL-21 mediated STAT3 signaling and IL-21 production in systemic lupus erythematosus.

Arthritis research & therapy

Rasmussen TK,Andersen T,Bak RO,Yiu G,Sørensen CM,Stengaard-Pedersen K,Mikkelsen JG,Utz PJ,Holm CK,Deleuran B

12721942 was used in flow cytometry to investigate the effect of miRNA-155 in IL-21 signaling in white cells and it role in systemic lupues erythematosus.

Tue Jun 09 00:00:00 EDT 2015

Impaired Phenotype and Function of T Follicular Helper Cells in HIV-1-Infected Children Receiving ART.

Medicine

Bekele Y,Amu S,Bobosha K,Lantto R,Nilsson A,Endale B,Gebre M,Aseffa A,Rethi B,Howe R,Chiodi F

12-7219 was used in Flow cytometry/Cell sorting to show that function and phenotype of Tfh cells, are impaired during HIV-1 infection in children, preventing adaptive B cell responses.

Wed Jul 01 00:00:00 EDT 2015

Galectin-9 and IL-21 mediate cross-regulation between Th17 and Treg cells during acute hepatitis C.

PLoS pathogens

Kared H,Fabre T,Bédard N,Bruneau J,Shoukry NH

12-7219 was used in Flow cytometry/Cell sorting to longitudinally analyse CD4 helper T cell function as well as the interaction between CD4 and CD8 T cells during acute HCV infection in individuals with spontaneous resolution vs persistent viremia.

Fri Jan 17 00:00:00 EST 2014

Increased frequency of circulating IL-21 producing Th-cells in patients with granulomatosis with polyangiitis (GPA).

Arthritis research & therapy

Abdulahad WH,Lepse N,Stegeman CA,Huitema MG,Doornbos-van der Meer B,Tadema H,Rutgers A,Limburg PC,Kallenberg CG,Heeringa P

12-7219 was used in Flow cytometry/Cell sorting to explore a possible role for IL-21 producing Th-cells in the immunopathogenesis of granulomatosis with polyangiitis.

Fri Nov 14 00:00:00 EST 2014

Engineering lymph node homing of ex vivo-expanded human natural killer cells via trogocytosis of the chemokine receptor CCR7.

Blood

Somanchi SS,Somanchi A,Cooper LJ,Lee DA

12-7219 was used in Flow cytometry/Cell sorting to explore the use of trogocytosis as a tool to transiently express CCR7 on expanded natural killer cells to enhance homing to lymph nodes.

Thu May 31 00:00:00 EDT 2012

Reprogramming CD19-specific T cells with IL-21 signaling can improve adoptive immunotherapy of B-lineage malignancies.

Cancer research

Singh H,Figliola MJ,Dawson MJ,Huls H,Olivares S,Switzer K,Mi T,Maiti S,Kebriaei P,Lee DA,Champlin RE,Cooper LJ

Sun May 15 00:00:00 EDT 2011

Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus.

Arthritis research & therapy

Dolff S,Abdulahad WH,Westra J,Doornbos-van der Meer B,Limburg PC,Kallenberg CG,Bijl M

12-7219 was used in Flow cytometry/Cell sorting to reveal a pivotal role of IL-21 in the pathogenesis of SLE.

Thu Dec 13 00:00:00 EST 2012

1,25-Dihydroxyvitamin D3 and IL-2 combine to inhibit T cell production of inflammatory cytokines and promote development of regulatory T cells expressing CTLA-4 and FoxP3.

Journal of immunology (Baltimore, Md. : 1950)

Jeffery LE,Burke F,Mura M,Zheng Y,Qureshi OS,Hewison M,Walker LS,Lammas DA,Raza K,Sansom DM

12-7219 was used in Flow cytometry/Cell sorting to assess the effects of the active form of vitamin D, 1,25-dihydroxyvitamin D(3) on human CD4(+)CD25(-) T cells.

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CX2; il 21; IL-21; ILN; Interleukin; Interleukin-21; interleukin-21 isoform; Interleukin21; Za11

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Gene Aliases: CVID11; IL-21; IL21; Za11

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UniProt ID: (Human) Q9HBE4

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Entrez Gene ID: (Human) 59067

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Function(s)

cytokine activity cytokine receptor binding interleukin-2 receptor binding interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), PE, eBioscience™

IL-21 Antibody (12-7219-42) in Flow

Product Details

12-7219-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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