Invitrogen

IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor™ 660, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

8 References

View all (38) IL-21 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 8

IL-21 Antibody (50-7219-42) in Flow

Intracellular staining of Th17-polarized normal human peripheral blood cells restimulated with monensin alone (left) or PMA/Ionomycin and monensin for 5 hours (right) with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-21 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

IL-21 Antibody — Figure 6 T cell-expressed C5aR1 regulates in vitro induction of IL-21-producing CD4+ T cells. Representative flow plots for IL-21-producing CD4+ T cells and summarized data of naive B6 CD62LhiCD44loCD4+ T cells from WT and C5ar1-/- mice cultured with aCD3/CD28 (A) or allogeneic BALB/c splenic APCs (B) under Tfh cell-inducing conditions for 5 days (n = 3 per group, repeated twice with similar results). (C) Representative flow plots for IL-21-producing CD4+ T cells and summarized data of naive B6 CD62LhiCD44loCD4+ T cells from WT TEa or C5ar1-/- TEa TCR-transgenic mice cultured with (bxd)F1 APCs under Tfh cell-inducing conditions for 5 days (n = 3/group, repeated once with similar results). (D and E) Representative flow plots (D) and quantitation (E) for c-MAF expression from WT and C5ar1-/- mice cultured with aCD3/CD28 with or without C5aR1-A for 5 days (n = 4/group, repeated twice with similar results). (F-H) Representative flow cytometry plots (F) and quantification (G) of IL-21 producers and quantitation of intracellular c-MAF (H) within human CD4+ T cells stimulated for 5 days under the conditions shown, with or without human C5aR1-A. n = 3-4/group. Each experiment shown is representative of at least 3 independent experiments for each set of studies. *P < 0.05, **P < 0.01 by Student’s t test. {TM}

Product Details

50-7219-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio3A3-N2 (3A3-N2)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598202

Product Specific Information

Description: The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.

Applications Reported: This eBio3A3-N2 (3A3-N2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio3A3-N2 (3A3-N2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining for IL-21 has shown donor variability. stimulation times may vary depending on the donor and should be optimized. Increased staining levels have been observed on Th17-polarized normal human peripheral blood cells.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.

Cell

Rosain J,Neehus AL,Manry J,Yang R,Le Pen J,Daher W,Liu Z,Chan YH,Tahuil N,Türel Ö,Bourgey M,Ogishi M,Doisne JM,Izquierdo HM,Shirasaki T,Le Voyer T,Guérin A,Bastard P,Moncada-Vélez M,Han JE,Khan T,Rapaport F,Hong SH,Cheung A,Haake K,Mindt BC,Pérez L,Philippot Q,Lee D,Zhang P,Rinchai D,Al Ali F,Ahmad Ata MM,Rahman M,Peel JN,Heissel S,Molina H,Kendir-Demirkol Y,Bailey R,Zhao S,Bohlen J,Mancini M,Seeleuthner Y,Roelens M,Lorenzo L,Soudée C,Paz MEJ,González ML,Jeljeli M,Soulier J,Romana S,L'Honneur AS,Materna M,Martínez-Barricarte R,Pochon M,Oleaga-Quintas C,Michev A,Migaud M,Lévy R,Alyanakian MA,Rozenberg F,Croft CA,Vogt G,Emile JF,Kremer L,Ma CS,Fritz JH,Lemon SM,Spaan AN,Manel N,Abel L,MacDonald MR,Boisson-Dupuis S,Marr N,Tangye SG,Di Santo JP,Zhang Q,Zhang SY,Rice CM,Béziat V,Lachmann N,Langlais D,Casanova JL,Gros P,Bustamante J

Published figure using IL-21 monoclonal antibody (Product # 50-7219-42) in Flow Cytometry

Thu Feb 02 00:00:00 EST 2023

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

Published figure using IL-21 monoclonal antibody (Product # 50-7219-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

IL-22 production of effector CD4⁺ T-cells is altered in SLE patients.

European journal of medical research

Dolff S,Scharpenberg C,Specker C,Kribben A,Witzke O,Wilde B

50-7219 was used in Flow Cytometry to demonstrate that CD134+CD4+ T-cells are increased in the peripheral blood of patients with systemic lupus erythematosus as compared to healthy controls.

Mon Jul 22 00:00:00 EDT 2019

Recognition of microbial viability via TLR8 drives T_FH cell differentiation and vaccine responses.

Nature immunology

Ugolini M,Gerhard J,Burkert S,Jensen KJ,Georg P,Ebner F,Volkers SM,Thada S,Dietert K,Bauer L,Schäfer A,Helbig ET,Opitz B,Kurth F,Sur S,Dittrich N,Gaddam S,Conrad ML,Benn CS,Blohm U,Gruber AD,Hutloff A,Hartmann S,Boekschoten MV,Müller M,Jungersen G,Schumann RR,Suttorp N,Sander LE

50-7219-42 was used in Flow Cytometry to identify TLR8 as an important driver of TFH cell differentiation and a promising target for TFH cell-skewing vaccine adjuvants.

Sun Apr 01 00:00:00 EDT 2018

Effects of Anti-TNFα Treatment on Mucosal Expression of IL-17A, IL-21, and IL-22 and Cytokine-Producing T Cell Subsets in Crohn's Disease.

Mediators of inflammation

Dige A,Magnusson MK,Uhrenholt C,Rasmussen TK,Kragstrup T,Öhman L,Dahlerup J,Agnholt J

Published figure using IL-21 monoclonal antibody (Product # 50-7219-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

C5aR1 regulates T follicular helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans.

JCI insight

Verghese DA,Chun N,Paz K,Fribourg M,Woodruff TM,Flynn R,Hu Y,Xiong H,Zhang W,Yi Z,Du J,Blazar BR,Heeger PS

Published figure using IL-21 monoclonal antibody (Product # 50-7219-42) in Flow Cytometry

Thu Dec 20 00:00:00 EST 2018

Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.

Journal of immunology (Baltimore, Md. : 1950)

Zanin-Zhorov A,Weiss JM,Trzeciak A,Chen W,Zhang J,Nyuydzefe MS,Arencibia C,Polimera S,Schueller O,Fuentes-Duculan J,Bonifacio KM,Kunjravia N,Cueto I,Soung J,Fleischmann RM,Kivitz A,Lebwohl M,Nunez M,Woodson J,Smith SL,West RF,Berger M,Krueger JG,Ryan JL,Waksal SD

50721942 was used in flow cytometry to find that oral administration of a selective Rho-associated kinase 2 inhibitor, KD025, reduces psoriasis area in 46% of patients with psoriasis vulgaris

Mon May 15 00:00:00 EDT 2017

Reprogramming CD19-specific T cells with IL-21 signaling can improve adoptive immunotherapy of B-lineage malignancies.

Cancer research

Singh H,Figliola MJ,Dawson MJ,Huls H,Olivares S,Switzer K,Mi T,Maiti S,Kebriaei P,Lee DA,Champlin RE,Cooper LJ

Published figure using IL-21 monoclonal antibody (Product # 50-7219-42) in Flow Cytometry

Sun May 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CX2; il 21; IL-21; ILN; Interleukin; Interleukin-21; interleukin-21 isoform; Interleukin21; Za11

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Gene Aliases: CVID11; IL-21; IL21; Za11

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UniProt ID: (Human) Q9HBE4

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Entrez Gene ID: (Human) 59067

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Function(s)

cytokine activity cytokine receptor binding interleukin-2 receptor binding interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor™ 660, eBioscience™

IL-21 Antibody (50-7219-42) in Flow

Product Details

50-7219-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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