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Invitrogen
IL-22 Monoclonal Antibody (IL22JOP), Functional Grade, eBioscience™
Product Details
16-7222-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rhesus monkey
Published species
Human,
Mouse,
Non-human primate,
Rhesus monkey
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
IL22JOP
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2016695
Product Specific Information
Description: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22. IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 cells, NK cells, and other T cells. Compared to IL-6 or TGF beta, IL-23 can induce greater levels of IL-22 in in vitro-differentiated Th17 cells. This observation suggests that IL-22 may be secreted by more fully differentiated Th17 cells in vivo. Recently, it was demonstrated that IL-22 could protect hosts from bacterial infection of the lungs and gut. Moreover, it has been reported that anti-CD3/CD28-induced production of IL-22 by PBMCs was elevated significantly in asthma patients compared to control patients. Flow cytometric analysis also showed that the frequencies of IL-17+IL-22+ CD4 T cells were increased in PBMCs from patients with ankylosing spondylitis and rheumatoid arthritis.
IL22JOP is published to recognize rhesus IL-22.
Applications Reported: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22.
Applications Tested: The ND50 of IL22JOP, as determined by the inhibition of IL-10 induction in COLO205 cells by IL-22, is 10-40 ng/mL, in the presence of 0.2 ng/mL recombinant human or mouse IL-22. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons. IL-22 is produced mainly by activated T cells and NK cells. In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10. IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2. IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29. Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway. In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling. IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes. IL-22 has been described as an effector cytokine of the Th17 lineage. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated. The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes. IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors. IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses. IL-22 is upregulated in activated T cells. IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytokine Zcyto18; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-22; IL-22a; IL-TIF; IL-TIF alpha; ILN; ILTIF alpha; Interleukin; interleukin 10-related T cell-derived inducible factor; Interleukin-22; Interleukin-22a; Interleukin22
Gene Aliases: EGK_03903; IL-21; IL-22; IL-22a; IL-D110; IL-TIF; IL22; Il22a; ILTIF; ILTIFa; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18
UniProt ID: (Human) Q9GZX6, (Mouse) Q9JJY9
Entrez Gene ID: (Human) 50616, (Rhesus monkey) 718047, (Mouse) 50929
acute-phase response
inflammatory response
immune response
cell-cell signaling
positive regulation of JAK-STAT cascade
response to glucocorticoid
regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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