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IL-25 Antibody (500-P89-1MG) in WB
Western Blot: To detect Human IL-17E by Western Blot analysis IL-25 Polyclonal Antibody (Product # 500-P89-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17E is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P89-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-17E
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930840
Product Specific Information
AA Sequence of recombinant protein: MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17E. Anti-Human IL-17E-specific antibody was purified by affinity chromatography employing an immobilized Human IL-17E matrix.
Sandwich ELISA: To detect Human IL-17E by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17E (500-P89Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17E.
Western Blot: To detect Human IL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17E is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: il 17e; IL-17E; IL-25; ILN; Interleukin; Interleukin-17E; Interleukin-25
Gene Aliases: IL17E; IL25; UNQ3120/PRO10272
UniProt ID: (Human) Q9H293
Entrez Gene ID: (Human) 64806
inflammatory response to antigenic stimulus
inflammatory response
cell surface receptor signaling pathway
biological_process
response to fungus
response to nematode
eosinophil differentiation
interleukin-13 production
interleukin-5 production
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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