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IL-25 Antibody (MA5-23893) in Flow
Flow cytometry of IL-25 in Tramp-C1 mouse prostate cancer cell line. Samples were incubated in IL-25 monoclonal antibody (Product # MA5-23893) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Product Details
MA5-23893
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2B
Class
Monoclonal
Type
Antibody
Clone
207702
Immunogen
E. coli-derived recombinant mouse IL-17E/IL-25 Val17-Ala169
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576228
Product Specific Information
In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-17E, rmIL-17, IL-17B, C, D, or F.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: il 17e; IL-17E; IL-25; ILN; Interleukin; interleukin 17E; Interleukin-17E; Interleukin-25
Gene Aliases: IL-17e; Il17e; Il25
UniProt ID: (Mouse) Q8VHH8
Entrez Gene ID: (Mouse) 140806
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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