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Invitrogen
IL-25R (IL-17RB) Monoclonal Antibody (MUNC33), PE, eBioscience™
FIGURE: 1 / 1
IL-25R (IL-17RB) Antibody (12-7361-82) in Flow
ILC2, but not CD4+ T cells, in the mesenteric lymph nodes express IL-25R. CD4+ T cells (CD45+, CD3+, CD4+) (left) and ILC2 (CD45+, CD19-, CD5-, CD11b-, CD11c-, NK1-1-, CD4-, CD90-2+, CD25+, CD127+, IL-33R+) (right) were stained with 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.125 µg of Anti-Mouse IL-25R (IL-17RB) PE (purple histogram). Single, viable cells were us... View More
Product Details
12-7361-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MUNC33
Immunogen
IL-17RB-Fc fusion protein
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572658
Product Specific Information
Description: This MUNC33 monoclonal antibody reacts with mouse interleukin-25 receptor (IL-25R), also known as IL-17RB. IL-25R is a 55 kDa transmembrane protein that dimerizes with IL-17RA to form the functional receptor for IL-25 (IL-17E). IL-25R is detectable in various tissues, including the lung and gastrointestinal tract and exists as both membrane bound and soluble form. IL-25/IL-25R interaction has been shown to activate the NF-kB, MAPK, and JNK pathways as well as gene transcription by STAT6, GATA-3, and NFATc1. IL-25R signals in T helper 2 (Th2) cells, type 2 innate lymphoid (ILC2) cells and some iNKT cells to promote differentiation and effector function. Whereas IL-25-deficient mice have an impaired Th2 response to parasitic helminth infection, elevated expression of IL-25 and IL-25R is observed in patients with chronic asthma and atopic dermatitis.
Applications Reported: This MUNC33 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MUNC33 antibody has been tested by flow cytometirc analysis of mouse lymph node cells or IL-25R-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E. IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth. IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8. IL-17Rh1 mRNA is highly expressed in Kidney and liver and at moderate level in small intestine, colon, and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: ecotropic viral integration site 27; IL-17 receptor B; IL-17 receptor homolog 1; IL-17B receptor; IL-17ER; IL-17RB; interleukin 17B receptor; Interleukin-17 receptor B; Interleukin-17B receptor; MGC138900; MGC138901; MGC5245; ZCYTO7
Gene Aliases: Evi27; IL-17ER; IL-17Rh1; Il17br; Il17rb; IL17RH1
UniProt ID: (Mouse) Q9JIP3
Entrez Gene ID: (Mouse) 50905
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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