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IL-27 Antibody (MA5-23551) in Flow
Flow cytometry of IL-27 in RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in IL-27 monoclonal antibody (Product # MA5-23551) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
Product Details
MA5-23551
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
234205
Immunogen
CHO-derived recombinant mouse IL-27 (aa 29-234 of p28, aa 19-228 of EBI-3)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
Buffered saline solution with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610324
Product Specific Information
This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.
Target Information
IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Epstein-Barr virus-induced gene 3 protein homolog; IL-27 p28 subunit; IL-27 subunit alpha; IL-27 subunit beta; IL-27-A; IL-27B; IL27 p28; IL27-A; ILN; Interleukin; interleukin 30; Interleukin-27 subunit alpha; Interleukin-27 subunit beta; M-IL-27 (p28); MGC71873; p28
Gene Aliases: EBI-3; Ebi3; IL-27; IL-27p28; Il27; Il27a; Il27b; Il30; p28
UniProt ID: (Mouse) O35228, (Mouse) Q8K3I6
Entrez Gene ID: (Mouse) 50498, (Mouse) 246779
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