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IL-28A Antibody (MA5-44954) in WB
Western blot analysis of IL-28A in different cell lysates (10 µg/Lane). Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in IL-28A Monoclonal antibody (Product # MA5-44954) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:20,000 for 1 hour at room temperature. Lane 1: MCF-7 cell lysate; Lane 2: 293 cell lysate. Predic... View More
Product Details
MA5-44954
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
A5D7
Immunogen
Synthetic peptide within human IL28A aa 110-160.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931410
Target Information
IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine Zcyto20; IFN-lambda-2; IL-28A; ILN; Interferon lambda-2; interferon, lambda 2; interferon-lambda 2; Interleukin; interleukin 28A (interferon, lambda 2); Interleukin-28A
Gene Aliases: IFNL2; IL-28A; IL28A; ZCYTO20
UniProt ID: (Human) Q8IZJ0
Entrez Gene ID: (Human) 282616
Performance Guarantee
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