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IL-31 Antibody (500-P249-1MG) in WB
Western Blot: To detect Human IL-31 by Western Blot analysis IL-31 Polyclonal Antibody (Product # 500-P249-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-31 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P249-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-31
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930051
Product Specific Information
AA Sequence of recombinant protein: SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-31. Anti-Human IL-31-specific antibody was purified by affinity chromatography employing an immobilized Human IL-31 matrix.
Sandwich ELISA: To detect Human IL-31 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-31 (500-P249Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-31.
Western Blot: To detect Human IL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-31 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IL-31; ILN; Interleukin; Interleukin-31
Gene Aliases: IL-31; IL31
UniProt ID: (Human) Q6EBC2
Entrez Gene ID: (Human) 386653
Performance Guarantee
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