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Product Details
PA5-20397
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 19 amino acid peptide from near the amino terminus of human IL-33.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11153521
Product Specific Information
Despite its predicted molecular weight, IL-33 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is human lymph node tissue lysate.
PA5-20397 can be used with blocking peptide PEP-0514.
Target Information
IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DVS27-related protein; growth stimulation-expressed; H-IL-33; homolog of mouse growth stimulation-expressed; IL-1F11; IL-33; ILN; Interleukin; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 family member 11; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin33; NF-HEV; nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules; Protein ST2; RP11-575C20.2
Gene Aliases: C9orf26; DER4; DVS27; FIT-1; IL1F11; IL1RL1; IL33; IL33R; NF-HEV; NFEHEV; NFHEV; ST2; ST2L; ST2V; T1
UniProt ID: (Human) Q01638, (Human) O95760
Entrez Gene ID: (Human) 9173, (Human) 90865
negative regulation of T-helper 1 type immune response
immune response
signal transduction
negative regulation of cell proliferation
negative regulation of interferon-gamma production
positive regulation of interleukin-5 production
interleukin-33-mediated signaling pathway
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
positive regulation of chemokine secretion
cellular response to peptide
negative regulation of leukocyte migration
positive regulation of type 2 immune response
transcription, DNA-templated
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of interleukin-13 production
positive regulation of interleukin-4 production
positive regulation of interleukin-6 production
type 2 immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of immunoglobulin secretion
negative regulation of immunoglobulin secretion
defense response to virus
extrinsic apoptotic signaling pathway
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