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Invitrogen
IL-33R (ST2) Monoclonal Antibody (hIL33Rcap), APC, eBioscience™
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
IL-33R (ST2) Antibody (17-9338-42) in Flow
LAD2 cells were stained with Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (blue histogram) or IL-33R (ST2) Monoclonal Antibody, APC (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
17-9338-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
hIL33Rcap
Immunogen
Extracellular domain of human IL-33R protein
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762446
Product Specific Information
Description: This hIL33Rcap monoclonal antibody recognizes human IL-33R, also known as ST2. This antibody binds both, the membrane bound form (ST2L) and the soluble form (ST2S). This antibody is suitable for both surface and intracellular staining.
This hIL33Rcap clone is also used as a capture antibody in the IL-33R (ST2) Human ProcartaPlex™ Simplex Kit (Product # EPX010-12231-901)
Applications Reported: This hIL33Rcap antibody has been reported for use in flow cytometric analysis.
Applications Tested: This hIL33Rcap antibody has been pre-diluted and tested by flow cytometric analysis of LAD2 cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Target Information
IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; IL-1RL1; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 receptor-like 1; Interleukin1 receptor like 1; Protein ST2; sIL 33R; soluble IL 33 Receptor; soluble IL 33R
Gene Aliases: DER4; FIT-1; IL1RL1; IL33R; ST2; ST2L; ST2V; T1
UniProt ID: (Human) Q01638
Entrez Gene ID: (Human) 9173
negative regulation of T-helper 1 type immune response
immune response
signal transduction
negative regulation of cell proliferation
negative regulation of interferon-gamma production
positive regulation of interleukin-5 production
interleukin-33-mediated signaling pathway
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
positive regulation of chemokine secretion
cellular response to peptide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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