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Invitrogen
IL-33R (ST2) Monoclonal Antibody (hIL33Rcap), Brilliant Violet™ 421, eBioscience™
FIGURE: 1 / 1
IL-33R (ST2) Antibody (404-9338-42) in Flow
LAD2 cells were cultured for 3-4 weeks in StemPro™-34 SFM (1X) (Product # 10639011) and treated with Human SCF Recombinant Protein (Product # 300-07-100UG). Cells were then stained with Mouse IgG1 kappa Isotype Control, Brilliant Violet 421 (Product # 404-4714-81) (blue histogram) or IL-33R (ST2) Monoclonal Antibody, Brilliant Violet 421 (purple histogram). Viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
404-9338-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
hIL33Rcap
Immunogen
Extracellular domain of human IL-33R protein
Conjugate
Excitation/Emission Max
406/423 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Description: This hIL33Rcap monoclonal antibody recognizes human IL-33R, also known as ST2. This antibody binds both, the membrane bound form (ST2L) and the soluble form (ST2S). This antibody is suitable for both surface and intracellular staining.
This hIL33Rcap clone is also used as a capture antibody in the IL-33R (ST2) Human ProcartaPlex™ Simplex Kit (Product # EPX010-12231-901).
Applications Reported: This hIL33Rcap antibody has been reported for use in flow cytometric analysis.
Applications Tested: This hIL33Rcap antibody has been pre-diluted and tested by flow cytometric analysis of LAD2. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
Target Information
IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; IL-1RL1; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 receptor-like 1; Interleukin1 receptor like 1; Protein ST2; sIL 33R; soluble IL 33 Receptor; soluble IL 33R
Gene Aliases: DER4; FIT-1; IL1RL1; IL33R; ST2; ST2L; ST2V; T1
UniProt ID: (Human) Q01638
Entrez Gene ID: (Human) 9173
negative regulation of T-helper 1 type immune response
immune response
signal transduction
negative regulation of cell proliferation
negative regulation of interferon-gamma production
positive regulation of interleukin-5 production
interleukin-33-mediated signaling pathway
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
positive regulation of chemokine secretion
cellular response to peptide
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