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Invitrogen
IL-4 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
IL-4 Antibody (PA5-47853) in ICC/IF
Immunocytochemistry analysis of IL-4 in immersion fixed porcine peripheral blood mononuclear cells (PBMCs). Samples were incubated in IL-4 polyclonal antibody (Product # PA5-47853) using a dilution of 5 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47853
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant porcine IL-4 His25-Cys133
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608125
Product Specific Information
In ELISAs, this antibody shows less than 2% cross-reactivity with recombinant feline IL-4 and less than 0.5% cross-reactivity with recombinant human IL-4, recombinant canine IL-4, recombinant cotton rat IL-4, recombinant mouse IL-4, and recombinant rat IL-4.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell stimulatory factor 1; BSF-1; cytokine; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: IL4
UniProt ID: (Pig) Q04745
Entrez Gene ID: (Pig) 397225
positive regulation of protein phosphorylation
positive regulation of gene expression
positive regulation of macroautophagy
B cell activation
negative regulation of transcription, DNA-templated
positive regulation of JAK-STAT cascade
regulation of protein export from nucleus
positive regulation of establishment of endothelial barrier
negative regulation of complement-dependent cytotoxicity
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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