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Invitrogen
IL-4 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
IL-4 Antibody (PA5-47930) in Neu
Neutralization of IL-4 in TF-1 human erythroleukemic cell line. Samples were incubated in IL-4 polyclonal antibody (Product # PA5-47930) using a dilution of 50 ng/mL. Recombinant Canine IL-4 stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-4 is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-4 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 2-8 µg/mL.
Product Details
PA5-47930
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant canine IL-4 His25-His132
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609990
Product Specific Information
In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-4 is observed, 15% cross-reactivity with recombinant feline IL-4 and recombinant porcine IL-4 is observed and less than 1% cross-reactivity with recombinant cotton rat IL-4, recombinant mouse IL-4, and recombinant rat IL-4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.01 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell stimulatory factor 1; BSF-1; cytokine; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: IL-4; IL4
UniProt ID: (Dog) O77762
Entrez Gene ID: (Dog) 403785
positive regulation of macroautophagy
positive regulation of B cell proliferation
positive regulation of T cell proliferation
B cell activation
regulation of phosphorylation
negative regulation of apoptotic process
positive regulation of MHC class II biosynthetic process
negative regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
regulation of immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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