Invitrogen

IL-4 Monoclonal Antibody (1D11)

FIGURE: 1 / 1

IL-4 Antibody (MM450C) in ELISA

Sandwich ELISA analysis of mouse IL-4 was performed using a Mouse IL-4 Colorimetric ELISA kit (Product # EMIL4) by loading 50 µL per well of Mouse IL-4 Recombinant Protein (Product # SMIL4) in dodecuplicate at 375, 75, 15 and 0 pg/mL across a 2 µg/mL rat anti-mouse IL-4 (Product # MM450C) pre-coated plate and incubating for 2 hours at 37°C. The plate was washed, then incubated with 100 µL per well of an HRP-conjugated rat anti-mouse IL-4 antibody at a concentration of 0.4 µg/mL for 1 hour at 37°C. Detection was performed using 1-Step Ultra... View More

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Product Details

MM450C

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 25 publications 25 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1D11

Immunogen

Recombinant mouse IL-4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223530

Product Specific Information

MM450C targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples. Does not bind to human IL-4.

The MM450C immunogen is recombinant mouse IL-4.

MM450C detects IL-4 which has a predicted molecular weight of approximately 14 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

The MM450C mouse IL4 antibody (clone 1D11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM450DB (biotinylated conjugate of clone 24G2). Typical dilutions for sandwich ELISA include 1-5 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM450C (clone 1D11) and biotinylated antibody MM450DB (clone 24G2) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Treadmill exercise induces murine cardiac allograft survival and generates regulatory T cell.

Transplant international : official journal of the European Society for Organ Transplantation

Uchiyama M,Jin X,Yin E,Shimokawa T,Niimi M

MM450C was used in ELISA to analyze induction of murine cardiac allograft survival and regulatory T cells from treadmill exercise

Sun Mar 01 00:00:00 EST 2015

Inchingorei-san (TJ-117) and Artemisiae Capillaris Herba Induced Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice.

Evidence-based complementary and alternative medicine : eCAM

Jin X,Uchiyama M,Zhang Q,Hirai T,Niimi M

MM450C was used in ELISA to examine the effects of Inchingorei-san treatment on the alloimmune responses in a murine model of cardiac allograft transplantation

Thu Aug 23 00:00:00 EDT 2012

Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells.

Journal of cardiothoracic surgery

Uchiyama M,Jin X,Zhang Q,Hirai T,Amano A,Bashuda H,Niimi M

MM450C was used in ELISA to assess the effect of sounds on alloimmune responses in a murine model of cardiac allograft transplantation

Fri Mar 23 00:00:00 EDT 2012

Induction of robust diabetes resistance and prevention of recurrent type 1 diabetes following islet transplantation by gene therapy.

Journal of immunology (Baltimore, Md. : 1950)

Tian C,Ansari MJ,Paez-Cortez J,Bagley J,Godwin J,Donnarumma M,Sayegh MH,Iacomini J

MM450C was used in ELISA to determine whether the programmed death-1-programmed death ligand-1 pathway precipitates type 1 diabetes in young NOD mice

Thu Nov 15 00:00:00 EST 2007

ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation.

The Journal of clinical investigation

Boldogh I,Bacsi A,Choudhury BK,Dharajiya N,Alam R,Hazra TK,Mitra S,Goldblum RM,Sur S

MM450C was used in ELISA to investigate the involvement of pollen NADPH oxidase in allergic airway inflammation

Mon Aug 01 00:00:00 EDT 2005

T cytotoxic-1 CD8+ T cells are effector cells against pneumocystis in mice.

Journal of immunology (Baltimore, Md. : 1950)

McAllister F,Steele C,Zheng M,Young E,Shellito JE,Marrero L,Kolls JK

MM450C was used in ELISA to investigate the role of T cytotoxic-1 CD8+ T cells in protection again pneumocystis

Thu Jan 15 00:00:00 EST 2004

Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen.

Transplantation

Aramaki O,Shirasugi N,Takayama T,Shimazu M,Kitajima M,Ikeda Y,Azuma M,Okumura K,Yagita H,Niimi M

MM450C was used in ELISA to determine the roles of PD-1 and its ligands in the induction of regulatory cells by intratracheal delivery of alloantigen

Thu Jan 15 00:00:00 EST 2004

Stimulation of interleukin-10 production by acidic beta-lactoglobulin-derived peptides hydrolyzed with Lactobacillus paracasei NCC2461 peptidases.

Clinical and diagnostic laboratory immunology

Prioult G,Pecquet S,Fliss I

MM450C was used in ELISA to investigate the induction of interleukin-10 production by acidic beta-lactoglobulin-derived peptides

Mon Mar 01 00:00:00 EST 2004

Enhanced mucosal and systemic immune responses to Helicobacter pylori antigens through mucosal priming followed by systemic boosting immunizations.

Immunology

Vajdy M,Singh M,Ugozzoli M,Briones M,Soenawan E,Cuadra L,Kazzaz J,Ruggiero P,Peppoloni S,Norelli F,del Giudice G,O'Hagan D

MM450C was used in ELISA to detect anti-CagA and anti-NAP cytokine-secreting cells

Mon Sep 01 00:00:00 EDT 2003

The levels and patterns of cytokines produced by CD4 T lymphocytes of BALB/c mice infected with Leishmania major by inoculation into the ear dermis depend on the infectiousness and size of the inoculum.

Infection and immunity

Lang T,Courret N,Colle JH,Milon G,Antoine JC

MM450C was used in ELISA to investigate the cytokine profile after Leishmania major inoculation

Thu May 01 00:00:00 EDT 2003

Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.

Journal of immunology (Baltimore, Md. : 1950)

Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P

MM450C was used in ELISA to study the vaccination against respiratory syncytial virus

Sun Sep 15 00:00:00 EDT 2002

The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.

Surgery

Zarzaur BL,Wu Y,Fukatsu K,Johnson CD,Kudsk KA

MM450C was used in ELISA to study the interleukin levels in the mice with various diets

Tue Jan 01 00:00:00 EST 2002

The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo.

Journal of bacteriology

Smith RS,Harris SG,Phipps R,Iglewski B

MM450C was used in ELISA to investigate the effect of 3O-C12-HSL for inflammation induction in mice

Fri Feb 01 00:00:00 EST 2002

Murine CD4+ T lymphocyte subsets and host defense against Pneumocystis carinii.

The Journal of infectious diseases

Shellito JE,Tate C,Ruan S,Kolls J

MM450C was used in ELISA to investigate the murine immunoresponse against Pneumocystis carinii

Thu Jun 01 00:00:00 EDT 2000

Oral administration of chitin down-regulates serum IgE levels and lung eosinophilia in the allergic mouse.

Journal of immunology (Baltimore, Md. : 1950)

Shibata Y,Foster LA,Bradfield JF,Myrvik QN

MM450C was used in ELISA to investigate the effect of chitin administration on serum IgE levels and lung eosinophilia

Tue Feb 01 00:00:00 EST 2000

C57BL/6 mice are more susceptible to antigen-induced pulmonary eosinophilia than BALB/c mice, irrespective of systemic T helper 1/T helper 2 responses.

Immunology

Morokata T,Ishikawa J,Ida K,Yamada T

MM450C was used in ELISA to investigate the strain-specific immune responses in mice

Mon Nov 01 00:00:00 EST 1999

Repeated administration of synthetic oligodeoxynucleotides expressing CpG motifs provides long-term protection against bacterial infection.

Infection and immunity

Klinman DM,Conover J,Coban C

MM450C was used in ELISA to investigate the protective effect of synthetic oligodeoxynucleotides against bacterial infection

Mon Nov 01 00:00:00 EST 1999

Sex-determined resistance against Leishmania mexicana is associated with the preferential induction of a Th1-like response and IFN-gamma production by female but not male DBA/2 mice.

Immunology and cell biology

Satoskar A,Al-Quassi HH,Alexander J

MM450C was used in ELISA to study the sex-specific immune resistance against Leishmania mexicana and its mechanism

Wed Apr 01 00:00:00 EST 1998

Mice with STAT6-targeted gene disruption develop a Th1 response and control cutaneous leishmaniasis.

Journal of immunology (Baltimore, Md. : 1950)

Stamm LM,Räisänen-Sokolowski A,Okano M,Russell ME,David JR,Satoskar AR

MM450C was used in ELISA to investigate the effect of STAT6 for a Th1-like response and on cutaneous L. mexicana infection in mice

Tue Dec 01 00:00:00 EST 1998

Topical FK506 suppresses cytokine and costimulatory molecule expression in epidermal and local draining lymph node cells during primary skin immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Homey B,Assmann T,Vohr HW,Ulrich P,Lauerma AI,Ruzicka T,Lehmann P,Schuppe HC

MM450C was used in flow cytometry to investigate the changes of gene expression by FK506 stimulation during primary skin immune responses

Mon Jun 01 00:00:00 EDT 1998

Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A

MM450C was used in ELISA to study the mechanism for T cell differentiation

Tue Sep 15 00:00:00 EDT 1998

Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.

Journal of immunology (Baltimore, Md. : 1950)

Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH

MM450C was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses

Thu Oct 15 00:00:00 EDT 1998

Induction of neonatal tolerance by plasmid DNA vaccination of mice.

The Journal of clinical investigation

Mor G,Yamshchikov G,Sedegah M,Takeno M,Wang R,Houghten RA,Hoffman S,Klinman DM

MM450C was used in ELISA to investigate the induction of neonatal tolerance by plasmid DNA vaccination of mice

Sun Dec 15 00:00:00 EST 1996

Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.

European journal of immunology

Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G

MM450C was used in blocking/activating experiment to investigate the role of interleukin 12 in Th1 cell function

Fri Mar 01 00:00:00 EST 1996

Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.

Journal of immunology (Baltimore, Md. : 1950)

Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E

MM450C was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation

Mon Jul 15 00:00:00 EDT 1996

T-helper type-1-dominated lymph node responses induced in C57BL/6 mice by optimally irradiated cercariae of Schistosoma mansoni are down-regulated after challenge infection.

Immunology

Pemberton RM,Wilson RA

MM450C was used in ELISA to investigate the immune response against Schistosoma mansoni

Wed Feb 01 00:00:00 EST 1995

Down-regulation of Th2 cell-mediated murine peritoneal eosinophilia by antiallergic agents.

Life sciences

Yamaya H,Basaki Y,Togawa M,Kojima M,Kiniwa M,Matsuura N

MM450C was used in ELISA to study the role of antiallergic agents in TH2 cell-mediated murine peritoneal eosinophilia

Thu May 25 00:00:00 EDT 1995

Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes.

Journal of immunology (Baltimore, Md. : 1950)

Finkelman FD,Madden KB,Morris SC,Holmes JM,Boiani N,Katona IM,Maliszewski CR

MM450C was used in immunocytochemistry to study the influence of anti-cytokine antibodies on the therapeutic effect of cytokine injection

Sun Aug 01 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

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IL-4 Monoclonal Antibody (1D11)

IL-4 Antibody (MM450C) in ELISA

Product Details

MM450C

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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