Proteintech

IL-4 Monoclonal Antibody (1G2C5)

IL-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

IL-4 Antibody (66142-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 66142-1-IG ( IL-4 antibody at dilution of 1:50 (under 40x lens).

IL-4 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

66142-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1G2C5

Immunogen

IL-4 Fusion Protein Ag9940 (24-153 aa encoded by BC070123)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.74 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GHKCDITLQ EIIKTLNSLT EQKTLCTELT VTDIFAASKN TTEKETFCRA ATVLRQFYSH HEKDTRCLGA TAQQFHRHKQ LIRFLKRLDR NLWGLAGLNS CPVKEANQST LENFLERLKT IMREKYSKCS S (24-153 aa encoded by BC070123)

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Journal of orthopaedic research : official publication of the Orthopaedic Research Society

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Thu Jun 01 00:00:00 EDT 2023

Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice.

Frontiers in immunology

Li X,Ye H,Su T,Hu C,Huang Y,Fu X,Zhong Z,Du X,Zheng Y

Mon May 29 00:00:00 EDT 2023

Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages.

Journal of translational medicine

Lu Y,Zhang H,Pan H,Zhang Z,Zeng H,Xie H,Yin J,Tang W,Lin R,Zeng C,Cai D

Mon May 22 00:00:00 EDT 2023

Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages.

Journal of translational medicine

Lu Y,Zhang H,Pan H,Zhang Z,Zeng H,Xie H,Yin J,Tang W,Lin R,Zeng C,Cai D

Mon May 22 00:00:00 EDT 2023

Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent Preeclampsia.

Cell transplantation

Liu H,Yu L,Ding Y,Peng M,Deng Y

Wed Jan 04 00:00:00 EST 2023

Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.

Evidence-based complementary and alternative medicine : eCAM

Fan R,Dai F,Zhang C,Zhou L,Qiu X,Li Y

Sat Jul 16 00:00:00 EDT 2022

Mesoporous silica nanoparticle-modified electrode arrays of cochlear implants for delivery of siRNA-TGFβ1 into the inner ear.

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Sat Oct 01 00:00:00 EDT 2022

One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing.

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Guo S,Yao M,Zhang D,He Y,Chang R,Ren Y,Guan F

Tue Feb 01 00:00:00 EST 2022

Intrahepatic eosinophilic proliferative phlebitis in Japanese black cattle indicate allergies involving mast cell tryptase-dependent activation.

Frontiers in veterinary science

Konnai M,Takahashi K,Machida Y,Michishita M,Ohkusu-Tsukada K

Wed Jan 11 00:00:00 EST 2023

Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing.

Frontiers in nutrition

Gong X,Liu L,Li X,Xiong J,Xu J,Mao D,Liu L

Tue Dec 06 00:00:00 EST 2022

Intravenous AAV9 administration results in safe and widespread distribution of transgene in the brain of mini-pig.

Frontiers in cell and developmental biology

Lin Y,Li C,Wang W,Li J,Huang C,Zheng X,Liu Z,Song X,Chen Y,Gao J,Wu J,Wu J,Tu Z,Lai L,Li XJ,Li S,Yan S

Sat Feb 11 00:00:00 EST 2023

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

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Fan TW,Daneshmandi S,Cassel TA,Uddin MB,Sledziona J,Thompson PT,Lin P,Higashi RM,Lane AN

Tue Nov 01 00:00:00 EDT 2022

IL-4 and CCR7 play an important role in the development of keloids in patients with a family history.

American journal of translational research

Shan M,Liu H,Hao Y,Meng T,Feng C,Song K,Wang Y

Sat Jul 16 00:00:00 EDT 2022

IL-4 and CCR7 play an important role in the development of keloids in patients with a family history.

American journal of translational research

Shan M,Liu H,Hao Y,Meng T,Feng C,Song K,Wang Y

Sat Jul 16 00:00:00 EDT 2022

IL-4 and CCR7 play an important role in the development of keloids in patients with a family history.

American journal of translational research

Shan M,Liu H,Hao Y,Meng T,Feng C,Song K,Wang Y

Sat Jul 16 00:00:00 EDT 2022

Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.

Molecular neurobiology

Tan Z,Yang G,Qiu J,Yan W,Liu Y,Ma Z,Li J,Liu J,Shan N

Sun May 01 00:00:00 EDT 2022

Circular RNA circWWC3 augments breast cancer progression through promoting M2 macrophage polarization and tumor immune escape via regulating the expression and secretion of IL-4.

Cancer cell international

Zheng Y,Ren S,Zhang Y,Liu S,Meng L,Liu F,Gu L,Ai N,Sang M

Mon Aug 22 00:00:00 EDT 2022

A Multicenter, Single-Blind, Case-Control, Immunohistochemical Study of Orbital Tissue in Thyroid Eye Disease.

Thyroid : official journal of the American Thyroid Association

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Interleukin-4 activates the PI3K/AKT signaling to promote apoptosis and inhibit the proliferation of granulosa cells.

Experimental cell research

Han Y,Yao R,Yang Z,Li S,Meng W,Zhang Y,Zhang Y,Luo H

Tue Mar 01 00:00:00 EST 2022

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Chitooligosaccharides alleviate hepatic fibrosis by regulating the polarization of M1 and M2 macrophages.

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Liu P,Li H,Gong J,Geng Y,Jiang M,Xu H,Xu Z,Shi J

Mon Jan 24 00:00:00 EST 2022

Chitooligosaccharides alleviate hepatic fibrosis by regulating the polarization of M1 and M2 macrophages.

Food & function

Liu P,Li H,Gong J,Geng Y,Jiang M,Xu H,Xu Z,Shi J

Mon Jan 24 00:00:00 EST 2022

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Li Y,Sheng Q,Zhang C,Han C,Bai H,Lai P,Fan Y,Ding Y,Dou X

Mon Feb 01 00:00:00 EST 2021

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Sun Mar 01 00:00:00 EST 2020

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Frontiers in cell and developmental biology

Wang T,Zhao N,Peng L,Li Y,Huang X,Zhu J,Chen Y,Yu S,Zhao Y

Tue Jan 05 00:00:00 EST 2021

Neural stem cells induce M2 polarization of macrophages through the upregulation of interleukin-4.

Experimental and therapeutic medicine

Ji Z,Jiang X,Li Y,Song J,Chai C,Lu X

Tue Dec 01 00:00:00 EST 2020

Beneficial Effects of Theta-Burst Transcranial Magnetic Stimulation on Stroke Injury via Improving Neuronal Microenvironment and Mitochondrial Integrity.

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Mon Jun 01 00:00:00 EDT 2020

Macrophage polarization in human gingival tissue in response to periodontal disease.

Oral diseases

Zhou LN,Bi CS,Gao LN,An Y,Chen F,Chen FM

Tue Jan 01 00:00:00 EST 2019

Macrophage polarization in human gingival tissue in response to periodontal disease.

Oral diseases

Zhou LN,Bi CS,Gao LN,An Y,Chen F,Chen FM

Tue Jan 01 00:00:00 EST 2019

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Rao S,Li Q,Wu H,Zhao M,Wang A,Zhang G,Li J,Lu L,Shi W,Lu Q

Sun Dec 01 00:00:00 EST 2019

Comparative Immunopathology of Cetacean morbillivirus Infection in Free-Ranging Dolphins From Western Mediterranean, Northeast-Central, and Southwestern Atlantic.

Frontiers in immunology

Díaz-Delgado J,Groch KR,Ressio R,Riskallah IPJ,Sierra E,Sacchini S,Quesada-Canales Ó,Arbelo M,Fernández A,Santos-Neto E,Ikeda J,de Carvalho RR,Azevedo AF,Lailson-Brito J,Flach L,Kanamura CT,Fernandes NCCA,Cogliati B,Centelleghe C,Mazzariol S,Di Renzo L,Di Francesco G,Di Guardo G,Catão-Dias JL

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Experimental neurology

Yang L,Tucker D,Dong Y,Wu C,Lu Y,Li Y,Zhang J,Liu TC,Zhang Q

Mon Jan 01 00:00:00 EST 2018

Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.

Experimental neurology

Yang L,Tucker D,Dong Y,Wu C,Lu Y,Li Y,Zhang J,Liu TC,Zhang Q

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Pre-transplant infusion of donor-derived dendritic cells maintained at the immature stage by sinomenine increases splenic Foxp3⁺ Tregs in recipient rats after renal allotransplantation.

Transplant immunology

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DNA and cell biology

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Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; cytokine; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Pig) Q04745, (Human) P05112

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Entrez Gene ID: (Pig) 397225, (Human) 3565

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Function(s)

interleukin-4 receptor binding growth factor activity cytokine activity protein binding interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-4 Monoclonal Antibody (1G2C5)

IL-4 Antibody (66142-1-IG) in IHC (P)

Product Details

66142-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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