Invitrogen

IL-4 Monoclonal Antibody (24G2)

Product Details

MM450D

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 4 publications 4 publications

ELISA (ELISA)

View 16 publications 16 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

24G2

Immunogen

Recombinant mouse IL-4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223531

Product Specific Information

MM450D targets IL-4 in WB applications and shows reactivity with mouse samples.

The MM450D immunogen is recombinant mouse IL-4.

MM450D detects IL-4 which has a predicted molecular weight of approximately 14 kDa.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inchingorei-san (TJ-117) and Artemisiae Capillaris Herba Induced Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice.

Evidence-based complementary and alternative medicine : eCAM

Jin X,Uchiyama M,Zhang Q,Hirai T,Niimi M

MM450D was used in ELISA to examine the effects of Inchingorei-san treatment on the alloimmune responses in a murine model of cardiac allograft transplantation

Thu Aug 23 00:00:00 EDT 2012

Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells.

Journal of cardiothoracic surgery

Uchiyama M,Jin X,Zhang Q,Hirai T,Amano A,Bashuda H,Niimi M

MM450D was used in ELISA to assess the effect of sounds on alloimmune responses in a murine model of cardiac allograft transplantation

Fri Mar 23 00:00:00 EDT 2012

Perforin-expressing cytotoxic cells contribute to chronic cardiomyopathy in Trypanosoma cruzi infection.

International journal of experimental pathology

Silverio JC,de-Oliveira-Pinto LM,da Silva AA,de Oliveira GM,Lannes-Vieira J

MM450D was used in immunohistochemistry - frozen section to assess the contribution of perforin in parasite control and chronic chagasic cardiomyopathy

Mon Feb 01 00:00:00 EST 2010

Induction of robust diabetes resistance and prevention of recurrent type 1 diabetes following islet transplantation by gene therapy.

Journal of immunology (Baltimore, Md. : 1950)

Tian C,Ansari MJ,Paez-Cortez J,Bagley J,Godwin J,Donnarumma M,Sayegh MH,Iacomini J

MM450D was used in ELISA to determine whether the programmed death-1-programmed death ligand-1 pathway precipitates type 1 diabetes in young NOD mice

Thu Nov 15 00:00:00 EST 2007

Role for CTLA-4 but not CD25+ T cells during Schistosoma mansoni infection of mice.

Parasite immunology

Walsh CM,Smith P,Fallon PG

MM450D was used in flow cytometry to study the regulation of CTLA-4+ and CD25+ T cells in schistosome infection

Fri Jun 01 00:00:00 EDT 2007

Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Mangan NE,van Rooijen N,McKenzie AN,Fallon PG

MM450D was used in flow cytometry to elucidate the mechanisms that regulate the inverse relationship between helminth infections and allergies

Sun Jan 01 00:00:00 EST 2006

A novel functional T cell hybridoma recognizes macrophage cell death induced by bacteria: a possible role for innate lymphocytes in bacterial infection.

Journal of immunology (Baltimore, Md. : 1950)

Kubota K

MM450D was used in flow cytometry to assess recognition of macrophage cell death induced by bacteria as a novel functional T cell hybridoma and its role for innate lymphocytes in bacterial infection

Thu Jun 15 00:00:00 EDT 2006

ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation.

The Journal of clinical investigation

Boldogh I,Bacsi A,Choudhury BK,Dharajiya N,Alam R,Hazra TK,Mitra S,Goldblum RM,Sur S

MM450D was used in ELISA to investigate the involvement of pollen NADPH oxidase in allergic airway inflammation

Mon Aug 01 00:00:00 EDT 2005

Humoral and cytokine response during protection of mice against secondary hydatidosis caused by Echinococcus granulosus.

Parasitology research

Al-Qaoud KM,Abdel-Hafez SK

MM450D was used in ELISA to induce protective immunity against secondary hydatidosis and elucidate the mechanisms of protection

Thu Dec 01 00:00:00 EST 2005

Helminth infection protects mice from anaphylaxis via IL-10-producing B cells.

Journal of immunology (Baltimore, Md. : 1950)

Mangan NE,Fallon RE,Smith P,van Rooijen N,McKenzie AN,Fallon PG

MM450D was used in flow cytometry to test if infection with Schistosoma mansoni alters the susceptibility of mice to anaphylaxis

Mon Nov 15 00:00:00 EST 2004

T cytotoxic-1 CD8+ T cells are effector cells against pneumocystis in mice.

Journal of immunology (Baltimore, Md. : 1950)

McAllister F,Steele C,Zheng M,Young E,Shellito JE,Marrero L,Kolls JK

MM450D was used in ELISA to investigate the role of T cytotoxic-1 CD8+ T cells in protection again pneumocystis

Thu Jan 15 00:00:00 EST 2004

Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen.

Transplantation

Aramaki O,Shirasugi N,Takayama T,Shimazu M,Kitajima M,Ikeda Y,Azuma M,Okumura K,Yagita H,Niimi M

MM450D was used in ELISA to determine the roles of PD-1 and its ligands in the induction of regulatory cells by intratracheal delivery of alloantigen

Thu Jan 15 00:00:00 EST 2004

The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo.

Journal of bacteriology

Smith RS,Harris SG,Phipps R,Iglewski B

MM450D was used in ELISA to investigate the effect of 3O-C12-HSL for inflammation induction in mice

Fri Feb 01 00:00:00 EST 2002

Immune response induced by airway sensitization after influenza A virus infection depends on timing of antigen exposure in mice.

Journal of virology

Yamamoto N,Suzuki S,Suzuki Y,Shirai A,Nakazawa M,Suzuki M,Takamasu T,Nagashima Y,Minami M,Ishigatsubo Y

MM450D was used in ELISA to characterize the airway immune response after influenza A virus infection

Mon Jan 01 00:00:00 EST 2001

Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.

Journal of immunology (Baltimore, Md. : 1950)

DeKruyff RH,Fang Y,Umetsu DT

MM450D was used in ELISA to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells

Sun Mar 01 00:00:00 EST 1998

Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A

MM450D was used in ELISA to study the mechanism for T cell differentiation

Tue Sep 15 00:00:00 EDT 1998

Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.

Journal of immunology (Baltimore, Md. : 1950)

Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH

MM450D was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses

Thu Oct 15 00:00:00 EDT 1998

Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.

European journal of immunology

Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G

MM450D was used in ELISA to investigate the role of interleukin 12 in Th1 cell function

Fri Mar 01 00:00:00 EST 1996

CD40-mediated stimulation contributes to lymphocyte proliferation, antibody production, eosinophilia, and mastocytosis during an in vivo type 2 response, but is not required for T cell IL-4 production.

Journal of immunology (Baltimore, Md. : 1950)

Lu P,Urban JF,Zhou XD,Chen SJ,Madden K,Moorman M,Nguyen H,Morris SC,Finkelman FD,Gause WC

MM450D was used in blocking/activating experiment to study the role of CD40 in immune response

Wed May 01 00:00:00 EDT 1996

Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.

Journal of immunology (Baltimore, Md. : 1950)

Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E

MM450D was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation

Mon Jul 15 00:00:00 EDT 1996

IgE receptor-positive non-B/non-T cells dominate the production of interleukin 4 and interleukin 6 in immunized mice.

Proceedings of the National Academy of Sciences of the United States of America

Aoki I,Kinzer C,Shirai A,Paul WE,Klinman DM

MM450D was used in ELISA to study the roles of IgE receptor-positive non-B/non-T cells in interleukin 4 and interleukin 6 production during immunization

Tue Mar 28 00:00:00 EST 1995

Down-regulation of Th2 cell-mediated murine peritoneal eosinophilia by antiallergic agents.

Life sciences

Yamaya H,Basaki Y,Togawa M,Kojima M,Kiniwa M,Matsuura N

MM450D was used in ELISA to study the role of antiallergic agents in TH2 cell-mediated murine peritoneal eosinophilia

Thu May 25 00:00:00 EDT 1995

Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes.

Journal of immunology (Baltimore, Md. : 1950)

Finkelman FD,Madden KB,Morris SC,Holmes JM,Boiani N,Katona IM,Maliszewski CR

MM450D was used in immunocytochemistry to study the influence of anti-cytokine antibodies on the therapeutic effect of cytokine injection

Sun Aug 01 00:00:00 EDT 1993

Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.

Journal of immunology (Baltimore, Md. : 1950)

Bogen SA,Fogelman I,Abbas AK

MM450D was used in immunohistochemistry to investigate the production of interleukin 2 and 4 and interferon gamma in mice immunized with protein antigens

Sat May 15 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (24G2)

Product Details

MM450D

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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