Invitrogen

IL-4 Monoclonal Antibody (24G2), Biotin

Product Details

MM450DB

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

24G2

Immunogen

Recombinant mouse IL-4

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_223604

Product Specific Information

MM450DB targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM450DB immunogen is recombinant mouse IL-4.

MM450DB detects IL-4 which has a predicted molecular weight of approximately 14 kDa.

The MM450DB mouse IL4 antibody (biotinylated conjugate of clone 24G2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM450C (clone 1D11). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody MM450DB (clone 24G2) and antibody MM450C (clone 1D11) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation.

The Journal of clinical investigation

Boldogh I,Bacsi A,Choudhury BK,Dharajiya N,Alam R,Hazra TK,Mitra S,Goldblum RM,Sur S

MM450DB was used in ELISA to investigate the involvement of pollen NADPH oxidase in allergic airway inflammation

Mon Aug 01 00:00:00 EDT 2005

T cytotoxic-1 CD8+ T cells are effector cells against pneumocystis in mice.

Journal of immunology (Baltimore, Md. : 1950)

McAllister F,Steele C,Zheng M,Young E,Shellito JE,Marrero L,Kolls JK

MM450DB was used in ELISA to investigate the role of T cytotoxic-1 CD8+ T cells in protection again pneumocystis

Thu Jan 15 00:00:00 EST 2004

Stimulation of interleukin-10 production by acidic beta-lactoglobulin-derived peptides hydrolyzed with Lactobacillus paracasei NCC2461 peptidases.

Clinical and diagnostic laboratory immunology

Prioult G,Pecquet S,Fliss I

MM450DB was used in ELISA to investigate the induction of interleukin-10 production by acidic beta-lactoglobulin-derived peptides

Mon Mar 01 00:00:00 EST 2004

Enhanced mucosal and systemic immune responses to Helicobacter pylori antigens through mucosal priming followed by systemic boosting immunizations.

Immunology

Vajdy M,Singh M,Ugozzoli M,Briones M,Soenawan E,Cuadra L,Kazzaz J,Ruggiero P,Peppoloni S,Norelli F,del Giudice G,O'Hagan D

MM450DB was used in ELISA to detect anti-CagA and anti-NAP cytokine-secreting cells

Mon Sep 01 00:00:00 EDT 2003

The levels and patterns of cytokines produced by CD4 T lymphocytes of BALB/c mice infected with Leishmania major by inoculation into the ear dermis depend on the infectiousness and size of the inoculum.

Infection and immunity

Lang T,Courret N,Colle JH,Milon G,Antoine JC

MM450DB was used in ELISA to investigate the cytokine profile after Leishmania major inoculation

Thu May 01 00:00:00 EDT 2003

Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.

Journal of immunology (Baltimore, Md. : 1950)

Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P

MM450DB was used in ELISA to study the vaccination against respiratory syncytial virus

Sun Sep 15 00:00:00 EDT 2002

The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.

Surgery

Zarzaur BL,Wu Y,Fukatsu K,Johnson CD,Kudsk KA

MM450DB was used in ELISA to study the interleukin levels in the mice with various diets

Tue Jan 01 00:00:00 EST 2002

Immune response induced by airway sensitization after influenza A virus infection depends on timing of antigen exposure in mice.

Journal of virology

Yamamoto N,Suzuki S,Suzuki Y,Shirai A,Nakazawa M,Suzuki M,Takamasu T,Nagashima Y,Minami M,Ishigatsubo Y

MM450DB was used in ELISA to characterize the airway immune response after influenza A virus infection

Mon Jan 01 00:00:00 EST 2001

Murine CD4+ T lymphocyte subsets and host defense against Pneumocystis carinii.

The Journal of infectious diseases

Shellito JE,Tate C,Ruan S,Kolls J

MM450DB was used in an ELISpot assay to study the T lymphocyte response to Pneumocystis carinii in mice.

Thu Jun 01 00:00:00 EDT 2000

IFN-gamma-inducing factor (IL-18) increases allergic sensitization, serum IgE, Th2 cytokines, and airway eosinophilia in a mouse model of allergic asthma.

Journal of immunology (Baltimore, Md. : 1950)

Wild JS,Sigounas A,Sur N,Siddiqui MS,Alam R,Kurimoto M,Sur S

MM450DB was used in ELISA to study the effect of interleukin-18 on allergic responses in a mouse asthma model

Wed Mar 01 00:00:00 EST 2000

Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.

Immunology

Bordmann G,Rudin W,Favre N

MM450DB was used in ELISA to investigate the effect of phosphatidylcholine immunization of mice for the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections

Fri May 01 00:00:00 EDT 1998

Mice with STAT6-targeted gene disruption develop a Th1 response and control cutaneous leishmaniasis.

Journal of immunology (Baltimore, Md. : 1950)

Stamm LM,Räisänen-Sokolowski A,Okano M,Russell ME,David JR,Satoskar AR

MM450DB was used in ELISA to investigate the effect of STAT6 for a Th1-like response and on cutaneous L. mexicana infection in mice

Tue Dec 01 00:00:00 EST 1998

Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.

Journal of immunology (Baltimore, Md. : 1950)

DeKruyff RH,Fang Y,Umetsu DT

MM450DB was used in ELISA to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells

Sun Mar 01 00:00:00 EST 1998

Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A

MM450DB was used in ELISA to study the mechanism for T cell differentiation

Tue Sep 15 00:00:00 EDT 1998

Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.

Journal of immunology (Baltimore, Md. : 1950)

Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH

MM450DB was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses

Thu Oct 15 00:00:00 EDT 1998

Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.

European journal of immunology

Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G

MM450DB was used in ELISA to investigate the role of interleukin 12 in Th1 cell function

Fri Mar 01 00:00:00 EST 1996

Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.

Journal of immunology (Baltimore, Md. : 1950)

Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E

MM450DB was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation

Mon Jul 15 00:00:00 EDT 1996

T-helper type-1-dominated lymph node responses induced in C57BL/6 mice by optimally irradiated cercariae of Schistosoma mansoni are down-regulated after challenge infection.

Immunology

Pemberton RM,Wilson RA

MM450DB was used in ELISA to investigate the immune response against Schistosoma mansoni

Wed Feb 01 00:00:00 EST 1995

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (24G2), Biotin

Product Details

MM450DB

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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