Invitrogen

IL-4 Monoclonal Antibody (BVD4-1D11)

2 Published Figures

12 References

View all (109) IL-4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (2)

FIGURE: 1 / 2

IL-4 Antibody (AMC0042) in Flow

Immunology 2003 - 4 Effect of TNF-alpha or LPS on preference of BC1 for Th differentiation. DO11.10 CD4 + T cells were co-cultured with unstimulated BC1 cells (iDC), BC1 cells matured by TNF-alpha or LPS (TNF/DC or LPS/DC) in the presence of a high- or low-dose OVA 323-339 peptide. The cells were stained by KJ1-26 mAb and monitored for intracellular staining of IFN-gamma and IL-4. (a) Dot plots gated by KJ1-26 positive cells... View More

Product Image Gallery

Product Details

AMC0042

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

View 7 publications 7 publications

Neutralization (Neu)

Assay-dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

BVD4-1D11

Immunogen

Recombinant mouse IL-4.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2536384

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IL-7Rα Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin.

Journal of immunology (Baltimore, Md. : 1950)

Kummola L,Ortutay Z,Chen X,Caucheteux S,Hämäläinen S,Aittomäki S,Yagi R,Zhu J,Pesu M,Paul WE,Junttila IS

AMC0042 was used in Flow cytometry/Cell sorting to show that murine ex vivo splenic DCs are unresponsive to TSLP, as they fail to phosphorylate STAT5, but in vitro overnight culture, especially in presence of IL-4, renders DCs responsive to both TSLP and IL-7.

Mon May 15 00:00:00 EDT 2017

Alternaria-induced release of IL-18 from damaged airway epithelial cells: an NF-κB dependent mechanism of Th2 differentiation?

PloS one

Murai H,Qi H,Choudhury B,Wild J,Dharajiya N,Vaidya S,Kalita A,Bacsi A,Corry D,Kurosky A,Brasier A,Boldogh I,Sur S

AMC0042 was used in ELISA to study the mechanism of alternaria-mediated damage in airway epithelial cells

Tue Jul 31 00:00:00 EDT 2012

Negative feedback regulation of T helper type 1 (Th1)/Th2 cytokine balance via dendritic cell and natural killer T cell interactions.

Blood

Minami K,Yanagawa Y,Iwabuchi K,Shinohara N,Harabayashi T,Nonomura K,Onoé K

AMC0042 was used in flow cytometry to examine the effects of the T helper type 1 and 2-cytokine environment on dendritic cell induction of natural killer T cell-mediated cytokine production

Thu Sep 01 00:00:00 EDT 2005

Social isolation stress exacerbates autoimmune disease in MRL/lpr mice.

Journal of neuroimmunology

Chida Y,Sudo N,Kubo C

AMC0042 was used in ELISA to investigate how isolation stress exacerbates autoimmune disease in MRL/lpr mice

Sat Jan 01 00:00:00 EST 2005

Humoral and cytokine response during protection of mice against secondary hydatidosis caused by Echinococcus granulosus.

Parasitology research

Al-Qaoud KM,Abdel-Hafez SK

AMC0042 was used in ELISA to induce protective immunity against secondary hydatidosis and elucidate the mechanisms of protection

Thu Dec 01 00:00:00 EST 2005

A transgenic mouse model for T-cell ignorance of a glial autoantigen.

Journal of autoimmunity

Cabarrocas J,Piaggio E,Zappulla JP,Desbois S,Mars LT,Lassmann H,Liblau RS

AMC0042 was used in ELISA to determine the fate of autoreactive CD4+T cells in HNT-TCR x GFAP-HA double transgenic mice

Sat May 01 00:00:00 EDT 2004

Arthropod-derived histamine-binding protein prevents murine allergic asthma.

Journal of immunology (Baltimore, Md. : 1950)

Couillin I,Maillet I,Vargaftig BB,Jacobs M,Paesen GC,Nuttall PA,Lefort J,Moser R,Weston-Davies W,Ryffel B

AMC0042 was used in ELISA to test if complete neutralization of histamine by an arthropod-derived, high affinity histamine-binding protein prevents allergic asthma

Wed Sep 01 00:00:00 EDT 2004

Assay system for the effect of prostaglandin E(2) in the determination of polarized cytokine production.

Methods in molecular biology (Clifton, N.J.)

Kuroda E,Yamashita U

AMC0042 was used in ELISA to examine the effect of prostaglandin E(2) on polarized cytokine production

Mon Feb 09 00:00:00 EST 2004

Mouse V alpha 14i natural killer T cells are resistant to cytokine polarization in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Matsuda JL,Gapin L,Baron JL,Sidobre S,Stetson DB,Mohrs M,Locksley RM,Kronenberg M

AMC0042 was used in flow cytometry to study V alpha 14i NKT cells.

Tue Jul 08 00:00:00 EDT 2003

Tumour necrosis factor-alpha but not lipopolysaccharide enhances preference of murine dendritic cells for Th2 differentiation.

Immunology

Kikuchi K,Yanagawa Y,Aranami T,Iwabuchi C,Iwabuchi K,Onoé K

Published figure using IL-4 monoclonal antibody (Product # AMC0042) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2003

Multiple Chlamydia pneumoniae antigens prime CD8+ Tc1 responses that inhibit intracellular growth of this vacuolar pathogen.

Journal of immunology (Baltimore, Md. : 1950)

Wizel B,Starcher BC,Samten B,Chroneos Z,Barnes PF,Dzuris J,Higashimoto Y,Appella E,Sette A

AMC0042 was used in ELISA to elucidate the role of CD8(+) T cells in immunity to Chlamydia pneumoniae.

Sun Sep 01 00:00:00 EDT 2002

Single cell analysis reveals that IL-4 receptor/Stat6 signaling is not required for the in vivo or in vitro development of CD4+ lymphocytes with a Th2 cytokine profile.

Journal of immunology (Baltimore, Md. : 1950)

Jankovic D,Kullberg MC,Noben-Trauth N,Caspar P,Paul WE,Sher A

AMC0042 was used in flow cytometry to report that IL-4R/Stat6 signaling controls the final frequency of Th2 lymphocytes, but is not essential for Th2 cell development.

Wed Mar 15 00:00:00 EST 2000

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

View more View less

Gene Aliases: BSF-1; Il-4; Il4

View more View less

UniProt ID: (Mouse) P07750

View more View less

Entrez Gene ID: (Mouse) 16189

View more View less

Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-4 Monoclonal Antibody (BVD4-1D11)

IL-4 Antibody (AMC0042) in Flow

Product Details

AMC0042

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!