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IL-4 Antibody (MA5-28465) in Flow
Flow cytometry of IL-4 in T lymphocytes. Samples were incubated with IL-4 monoclonal antibody (Product # MA5-28465). Image details are as follows: A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. B) Pseudocolor plots representing cytokines + (IL-17, TNF-alpha, IFN-gamma, TGF-beta and IL-4) CD4+ cells within gated lymphocyte... View More
Product Details
MA5-28465
Product Specifications
Species Reactivity
Bovine
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
CC303
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 5% sucrose, 1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745426
Product Specific Information
Reconstitute with 1 mL of distilled water.
Mouse anti Bovine Interleukin-4 antibody, clone CC303 recognizes bovine interleukin 4.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell stimulatory factor 1; BSF-1; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: BSF-1; IL-4; IL4
UniProt ID: (Bovine) P30367
Entrez Gene ID: (Bovine) 280824
positive regulation of macroautophagy
positive regulation of B cell proliferation
positive regulation of T cell proliferation
B cell activation
regulation of phosphorylation
negative regulation of apoptotic process
positive regulation of MHC class II biosynthetic process
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
regulation of immune response
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