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Leinco Technologies
IL-4 Monoclonal Antibody (MP4-25D2), Functional Grade, In Vivo GOLD™
Product Details
I-1163-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
MP4-25D2
Immunogen
CHO-expressed, recombinant human IL-4
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Specificity: Clone MP4-25D2 recognizes human IL-4.
Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥95% monomer by analytical SEC.
Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.
This antibody has been tested in the following applications: Western Blotting, Neutralization, Immunohistochemistry, CyTOF®.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra
Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4
UniProt ID: (Human) P05112
Entrez Gene ID: (Human) 3565
microglial cell activation
innate immune response in mucosa
positive regulation of defense response to virus by host
T-helper 1 cell lineage commitment
negative regulation of acute inflammatory response
negative regulation of chronic inflammatory response
chemotaxis
immune response
cellular defense response
female pregnancy
response to nutrient
cholesterol metabolic process
regulation of proton transport
negative regulation of epithelial cell migration
response to organic cyclic compound
B cell differentiation
positive regulation of B cell proliferation
B cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
response to cytokine
T-helper 2 cell cytokine production
type 2 immune response
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
regulation of phosphorylation
response to drug
positive regulation of tyrosine phosphorylation of Stat5 protein
defense response to protozoan
myeloid dendritic cell differentiation
negative regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of mast cell degranulation
negative regulation of nitric oxide biosynthetic process
T-helper 2 cell differentiation
positive regulation of chemokine biosynthetic process
connective tissue growth factor biosynthetic process
regulation of isotype switching
positive regulation of MHC class II biosynthetic process
response to ethanol
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
regulation of immune response
positive regulation of sequence-specific DNA binding transcription factor activity
retina development in camera-type eye
negative regulation of white fat cell proliferation
cellular response to mercury ion
positive regulation of mononuclear cell migration
dendritic cell differentiation
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of myoblast fusion
negative regulation of complement-dependent cytotoxicity
negative regulation of T-helper 17 cell differentiation
negative regulation of endothelial cell apoptotic process
positive regulation of eosinophil chemotaxis
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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