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Invitrogen
IL-5 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
IL-5 Antibody (PA5-47231) in ICC/IF
Immunocytochemistry analysis of IL-5 in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA. Samples were incubated in IL-5 polyclonal antibody (Product # PA5-47231) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surface.
Product Details
PA5-47231
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine IL-5 Phe20-Ser134
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606213
Product Specific Information
In Western blots, approximately 15% cross-reactivity with recombinant bovine IL-5, recombinant equine IL-5, and recombinant feline IL-5 is observed and less than 5% cross-reactivity with recombinant rat IL-5, recombinant mouse IL-5, and recombinant human IL-5 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Eosinophil differentiation factor; IL-5; ILN; Interleukin; interleukin 5 (colony-stimulating factor, eosinophil); Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: IL5
UniProt ID: (Dog) Q95J76
Entrez Gene ID: (Dog) 403790
immune response
signal transduction
positive regulation of transcription, DNA-templated
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of podosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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