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IL-5 Antibody (M551) in ELISA
Sandwich ELISA analysis of human IL-5 was performed by coating wells of a 96-well plate with 110 µL of a human IL-5 monoclonal antibody (Product # M551) in duplicate at 1, 2, 4, 5, 7, and 9 µg/mL for at least 12 hours. The plate was blocked with a proprietary blocking solution for 1-4 hours at room temperature, and 50 µL of mouse IL-5 recombinant protein (Product # SMIL5) was added in replicates of twelve at 400, 160, 64, 25.6, 10.24, and 0pg/mL for 1 hour at room temperature. The plate was washed and incubated 50 µL of a biotinylated IL-5... View More
Product Details
M551
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
5A10
Immunogen
Recombinant human IL-5
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_223574
Product Specific Information
M551 targets IL-5 in ELISA, and WB applications and shows reactivity with Human samples.
The M551 immunogen is recombinant human IL-5.
M551 detects IL-5 which has a predicted molecular weight of approximately 13 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The M551 IL5 antibody (clone 5A10) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M550B (biotinylated conjugate of clone 39D10). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody M551 (clone 5A10) and biotinylated antibody M550B (clone 39D10) have successfully been used in combination with recombinant IL5 protein SIL5 in ELISA applications.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell differentiation factor I; colony-stimulating factor, eosinophil; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: EDF; IL-5; IL5; TRF
UniProt ID: (Human) P05113
Entrez Gene ID: (Human) 3567
MAPK cascade
inflammatory response
immune response
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
positive regulation of B cell proliferation
positive regulation of GTPase activity
positive regulation of eosinophil differentiation
positive regulation of transcription, DNA-templated
positive regulation of JAK-STAT cascade
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of podosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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