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IL-5 Antibody (MA5-29367) in Neu
Cell Proliferation Induced by IL-5 and Neutralization by IL-5 Recombinant Rabbit Monoclonal Antibody (R013) (Product # MA5-29367). Human IL-5 stimulates proliferation in the TF-1 human erythroleukemic cell line. Proliferation elicited by Recombinant Human IL-5 (1.25 ng/mL) is neutralized by increasing concentrations of IL-5 Recombinant Rabbit Monoclonal Antibody (R013). The ND50is typically 2-8 ng/mL.
Product Details
MA5-29367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
R013
Immunogen
Recombinant Human IL-5/IL5/Interleukin 5 protein (Ile20-Ser134)
Conjugate
Form
Liquid
Concentration
2.67 mg/mL
Purification
Protein A
Storage buffer
Histidine/arginine, pH 6, with 120mM NaCl, 0.02% Tween 80
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785242
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human IL-5/IL5/Interleukin 5.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell differentiation factor I; colony-stimulating factor, eosinophil; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: EDF; IL-5; IL5; TRF
UniProt ID: (Human) P05113
Entrez Gene ID: (Human) 3567
MAPK cascade
inflammatory response
immune response
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
positive regulation of B cell proliferation
positive regulation of GTPase activity
positive regulation of eosinophil differentiation
positive regulation of transcription, DNA-templated
positive regulation of JAK-STAT cascade
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of podosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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