Invitrogen

IL-5 Monoclonal Antibody (TRFK5), PE, eBioscience™

FIGURE: 1 / 2

IL-5 Antibody (12-7052-82) in Flow

Normal human peripheral blood cells were polarized under Th2 conditions and then unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (Product # 00-4970-03) in the presence of Brefeldin A Solution (Product # 00-4506-51) (right). Cells were intracellularly stained with Anti-Human CD4 FITC (Product # 11-0048-42) and 0.125 µg of Anti-Human/Mouse IL-5 PE using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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Product Details

12-7052-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 21 publications 21 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TRFK5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_763587

Product Specific Information

Description: The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5). The TRFK5 antibody is a neutralizing antibody. mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.

Applications Reported: This TRFK5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This TRFK5 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

Cell reports

Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

12-7052 was used in Flow cytometry/Cell sorting to reveal the role of LAL in T cell alloresponse and pathogenicity and validates LAL as a target for controlling GVHD and tumor relapse after allo-HCT.

Tue Oct 27 00:00:00 EDT 2020

CD4⁺ T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection.

Cell reports

Wen J,Wang YT,Valentine KM,Dos Santos Alves RP,Xu Z,Regla-Nava JA,Ngono AE,Young MP,Ferreira LCS,Shresta S

12-7052 was used in Flow cytometry/Cell sorting to examine the ability of DENV/ZIKV-cross-reactive CD4+ T cells to protect against versus exacerbate ZIKV infection.

Tue Apr 28 00:00:00 EDT 2020

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

12-7052 was used in Flow cytometry/Cell sorting to suggest a revised model for ILC differentiation that redefines the cell-fate potential of helper-ILC-restricted Zbtb16+ ILCPs.

Tue Apr 16 00:00:00 EDT 2019

Ovalbumin induces natural killer cells to secrete Th2 cytokines IL‑5 and IL‑13 in a mouse model of asthma.

Molecular medicine reports

Chen Z,Wang L

12-7052-82 was used in Flow Cytometry to analyse the dynamic changes of NK cells and their subsets during the development of the ovalbumin (OVA)‚induced allergic airway response.

Mon Apr 01 00:00:00 EDT 2019

Dephosphorylated Polymerase I and Transcript Release Factor Prevents Allergic Asthma Exacerbations by Limiting IL-33 Release.

Frontiers in immunology

Ni Y,Hao J,Hou X,Du W,Yu Y,Chen T,Wei Z,Li Y,Zhu F,Wang S,Liang R,Li D,Lu Y,Liao K,Li B,Shi G

Mon Mar 21 00:00:00 EDT 2022

Bcl11b is essential for licensing Th2 differentiation during helminth infection and allergic asthma.

Nature communications

Lorentsen KJ,Cho JJ,Luo X,Zuniga AN,Urban JF,Zhou L,Gharaibeh R,Jobin C,Kladde MP,Avram D

12-7052 was used in Flow cytometry/Cell sorting to establish novel roles for Bcl11b in the regulatory loop that licenses Th2 program in vivo.

Thu Apr 26 00:00:00 EDT 2018

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

12-7052 was used in Flow cytometry/Cell sorting to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 EDT 2017

Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis.

The Journal of allergy and clinical immunology

Chen PC,Hsieh MH,Kuo WS,Kao HF,Hsu CL,Wang JY

12-7052 was used in Flow cytometry/Cell sorting to evaluate the effect of extensive water-soluble chitosan in allergic rhinitis.

Sun Oct 01 00:00:00 EDT 2017

Intestinal helminth infection induces highly functional resident memory CD4⁺ T cells in mice.

European journal of immunology

Steinfelder S,Rausch S,Michael D,Kühl AA,Hartmann S

12-7052 was used in Flow cytometry/Cell sorting to show that nonlymphoid compartments can serve as reservoirs for Th2 memory cells, and furthermore that innate effector function of Th2 memory cells is restricted to CD4⁺ memory T cells residing in the peritoneal cavity.

Wed Feb 01 00:00:00 EST 2017

Toxoplasma Co-infection Prevents Th2 Differentiation and Leads to a Helminth-Specific Th1 Response.

Frontiers in cellular and infection microbiology

Ahmed N,French T,Rausch S,Kühl A,Hemminger K,Dunay IR,Steinfelder S,Hartmann S

12-7052 was used in Flow cytometry/Cell sorting to conclude a previous T. gondii infection limits a helminth-specific Th2 immune response while promoting a shift toward a Th1-type immune response.

Mon Apr 02 00:00:00 EDT 2018

Lack of autophagy induces steroid-resistant airway inflammation.

The Journal of allergy and clinical immunology

Suzuki Y,Maazi H,Sankaranarayanan I,Lam J,Khoo B,Soroosh P,Barbers RG,James Ou JH,Jung JU,Akbari O

12-7052 was used in Flow cytometry/Cell sorting to examine the role of autophagy in lung inflammation and the pathogenesis of corticosteroid-resistant neutrophilic asthma.

Sun May 01 00:00:00 EDT 2016

Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung.

PloS one

De Grove KC,Provoost S,Verhamme FM,Bracke KR,Joos GF,Maes T,Brusselle GG

12-7052 was used in Flow cytometry/Cell sorting to study innate lymphoid cells in the human lung in healthy patients and those with chronic pulmonary disease.

Fri Jul 08 00:00:00 EDT 2016

Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region.

PloS one

Kim K,Kim N,Lee GR

12-7052 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms behind Th2 cytokine gene regulation.

Thu Jul 07 00:00:00 EDT 2016

The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors.

BMC hematology

Silveira AC,Santana MA,Ribeiro IG,Chaves DG,Martins-Filho OA

12-7052 was used in Flow cytometry/Cell sorting to understand immune response to FVIII in patients with HA who were either positive [HAα-FVIII(+)] or negative [HAα-FVIII(-)] for inhibitors.

Thu Feb 05 00:00:00 EST 2015

TNF superfamily member TL1A elicits type 2 innate lymphoid cells at mucosal barriers.

Mucosal immunology

Yu X,Pappu R,Ramirez-Carrozzi V,Ota N,Caplazi P,Zhang J,Yan D,Xu M,Lee WP,Grogan JL

12-7052 was used in an ELISA assay to demonstrate a key role for TL1A in promoting ILC2s at mucosal barriers.

Thu May 01 00:00:00 EDT 2014

Group V secretory phospholipase A2 is involved in macrophage activation and is sufficient for macrophage effector functions in allergic pulmonary inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Ohta S,Imamura M,Xing W,Boyce JA,Balestrieri B

12-7052 was used in Flow cytometry/Cell sorting to identify a unique function for gV-sPLA2 in the activation and recruitment of immune cells.

Sat Jun 15 00:00:00 EDT 2013

Thymic stromal lymphopoietin induces corticosteroid resistance in natural helper cells during airway inflammation.

Nature communications

Kabata H,Moro K,Fukunaga K,Suzuki Y,Miyata J,Masaki K,Betsuyaku T,Koyasu S,Asano K

12-7052 was used in Flow cytometry/Cell sorting to investigate the role of the IL-33/NH-cell axis in corticosteroid resistance.

Thu Jun 12 00:00:00 EDT 2014

Transcription factor RORα is critical for nuocyte development.

Nature immunology

Wong SH,Walker JA,Jolin HE,Drynan LF,Hams E,Camelo A,Barlow JL,Neill DR,Panova V,Koch U,Radtke F,Hardman CS,Hwang YY,Fallon PG,McKenzie AN

12-7052 was used in Flow cytometry/Cell sorting to study how nuocytes arose in the bone marrow and differentiated from common lymphoid progenitors, indicating they are a distinct, previously unknown member of the lymphoid lineage.

Sun Jan 22 00:00:00 EST 2012

Lack of IL-4 receptor expression on T helper cells reduces T helper 2 cell polyfunctionality and confers resistance in allergic bronchopulmonary mycosis.

Mucosal immunology

Müller U,Piehler D,Stenzel W,Köhler G,Frey O,Held J,Grahnert A,Richter T,Eschke M,Kamradt T,Brombacher F,Alber G

12-7052 was used in Flow cytometry/Cell sorting to study the function of the interleukin-4 receptor on T helper cells in pulmonary cryptococcosis.

Tue May 01 00:00:00 EDT 2012

Donor B cells in transplants augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like chronic graft-versus-host disease.

Journal of immunology (Baltimore, Md. : 1950)

Young JS,Wu T,Chen Y,Zhao D,Liu H,Yi T,Johnston H,Racine J,Li X,Wang A,Todorov I,Zeng D

12-7052 was used in Flow cytometry/Cell sorting to investigate the mechanisms whereby donor B cells augment chronic graft-versus-host disease (cGVHD) pathogenesis, showing that B cells augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like cGVHD.

Sun Jul 01 00:00:00 EDT 2012

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

12-7052 was used in Magnetic cell separation to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

Bronchial epithelial cells produce IL-5: implications for local immune responses in the airways.

Cellular immunology

Wu CA,Peluso JJ,Zhu L,Lingenheld EG,Walker ST,Puddington L

12-7052 was used in Immunofluorescence to investigate how IL-5 production by BEC can impact the microenvironment of the lung, modifying pathological and protective immune responses in the airways.

Wed Sep 01 00:00:00 EDT 2010

Pancreatic lipase-related protein 2 (PLRP2) induction by IL-4 in cytotoxic T lymphocytes (CTLs) and reevaluation of the negative effects of its gene ablation on cytotoxicity.

Journal of leukocyte biology

Alves BN,Leong J,Tamang DL,Elliott V,Edelnant J,Redelman D,Singer CA,Kuhn AR,Miller R,Lowe ME,Hudig D

12-7052 was used in Flow cytometry/Cell sorting to study how extracellular PLRP2 lipase is unable to increase the cytotoxicity lost through PLRP2 ablation, and the overall role of PLRP2 in CD8 T cells..

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF

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Gene Aliases: EDF; IL-5; IL5; TRF

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UniProt ID: (Human) P05113, (Mouse) P04401

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Entrez Gene ID: (Human) 3567, (Mouse) 16191

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Function(s)

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-5 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-5 Monoclonal Antibody (TRFK5), PE, eBioscience™

IL-5 Antibody (12-7052-82) in Flow

Product Details

12-7052-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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