Invitrogen

IL-5 Monoclonal Antibody (TRFK5), eBioscience™

FIGURE: 1 / 6

IL-5 Antibody (14-7052-81) in IS

Naive mouse splenocytes (SJL/J strain) were stimulated with 2 µg/mL ConA in HL-1 media for 48 hours (right well) in a Mouse IL-5 ELISPOT assay plate. Left well is medium alone control.

IL-5 Antibody in Radioimmune assays (RIA)

Product Image Gallery

Product Details

14-7052-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 4 publications 4 publications

ELISA (ELISA)

View 9 publications 9 publications

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Radioimmune Assays (RIA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Immunostaining (IS)

1-4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

TRFK5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468420

Product Specific Information

Description: The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5). The TRFK5 antibody is a neutralizing antibody. Mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.

Applications Reported: The TRFK5 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-5 bioactivity, IHC, and intracellular staining for flow cytometric analysis.

Applications Tested: The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-5 in combination with the biotin TRFK4 (13-7051) antibody for detection and recombinant mouse IL-5 (14-8051) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-5 in combination with the biotinylated JES1-5A10 (13-7059) antibody for detection and recombinant human IL-5 (14-80591) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

The Functional Grade purified TRFK5 antibody has been tested for neutralization of IL-5 bioactivity.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Il4ra-independent vaginal eosinophil accumulation following helminth infection exacerbates epithelial ulcerative pathology of HSV-2 infection.

Cell host & microbe

Chetty A,Darby MG,Vornewald PM,Martín-Alonso M,Filz A,Ritter M,McSorley HJ,Masson L,Smith K,Brombacher F,O'Shea MK,Cunningham AF,Ryffel B,Oudhoff MJ,Dewals BG,Layland LE,Horsnell WGC

Published figure using IL-5 monoclonal antibody (Product # 14-7052-81) in ELISA

Wed Apr 14 00:00:00 EDT 2021

Il4ra-independent vaginal eosinophil accumulation following helminth infection exacerbates epithelial ulcerative pathology of HSV-2 infection.

Cell host & microbe

Chetty A,Darby MG,Vornewald PM,Martín-Alonso M,Filz A,Ritter M,McSorley HJ,Masson L,Smith K,Brombacher F,O'Shea MK,Cunningham AF,Ryffel B,Oudhoff MJ,Dewals BG,Layland LE,Horsnell WGC

Published figure using IL-5 monoclonal antibody (Product # 14-7052-81) in ELISA

Wed Apr 14 00:00:00 EDT 2021

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

14-7052 was used in ELISA to study the retrograde traffic of linker for activation of T cells from the plasma membrane to the Golgi-trans-Golgi network.

Mon Apr 02 00:00:00 EDT 2018

Bcl11b is essential for licensing Th2 differentiation during helminth infection and allergic asthma.

Nature communications

Lorentsen KJ,Cho JJ,Luo X,Zuniga AN,Urban JF,Zhou L,Gharaibeh R,Jobin C,Kladde MP,Avram D

Published figure using IL-5 monoclonal antibody (Product # 14-7052-81) in Flow Cytometry

Thu Apr 26 00:00:00 EDT 2018

Intratracheal myriocin enhances allergen-induced Th2 inflammation and airway hyper-responsiveness.

Immunity, inflammation and disease

Edukulla R,Rehn KL,Liu B,McAlees JW,Hershey GK,Wang YH,Lewkowich I,Lindsley AW

14-7052 was used in an ELISA assay to evaluate the immune regulatory role of de novo ceramide generation in vitro and in vivo.

Thu Sep 01 00:00:00 EDT 2016

Concerted activity of IgG1 antibodies and IL-4/IL-25-dependent effector cells trap helminth larvae in the tissues following vaccination with defined secreted antigens, providing sterile immunity to challenge infection.

PLoS pathogens

Hewitson JP,Filbey KJ,Esser-von Bieren J,Camberis M,Schwartz C,Murray J,Reynolds LA,Blair N,Robertson E,Harcus Y,Boon L,Huang SC,Yang L,Tu Y,Miller MJ,Voehringer D,Le Gros G,Harris N,Maizels RM

14-7052 was used in Flow cytometry/Cell sorting to show that vaccine-induced immunity to intestinal helminths involves both IgG1 antibodies and IL-25-dependent Type 2 myeloid effector populations.

Sun Mar 01 00:00:00 EST 2015

Fiber composite slices for multiplexed immunoassays.

Biomicrofluidics

Kim J,Bae S,Song S,Chung K,Kwon S

14-7052 was used in Immuno-assay to describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads.

Wed Jul 01 00:00:00 EDT 2015

Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.

Infection and immunity

Dulek DE,Newcomb DC,Goleniewska K,Cephus J,Zhou W,Reiss S,Toki S,Ye F,Zaynagetdinov R,Sherrill TP,Blackwell TS,Moore ML,Boyd KL,Kolls JK,Peebles RS

14-7052 was used in Neutralization experiments to determine the effect of IL-4 and IL-13 on IL-17-dependent lung immune responses to acute bacterial infection.

Mon Sep 01 00:00:00 EDT 2014

The Escherichia coli heat-labile enterotoxin B subunit protects from allergic airway disease development by inducing CD4+ regulatory T cells.

Mucosal immunology

Donaldson DS,Apostolaki M,Bone HK,Richards CM,Williams NA

14-7052 was used in Flow cytometry/Cell sorting to demonstrate that EtxB modulates the differentiation of allergen-specific T cells causing inducible Treg induction and preventing disease.

Wed May 01 00:00:00 EDT 2013

Group 2 innate lymphoid cell production of IL-5 is regulated by NKT cells during influenza virus infection.

PLoS pathogens

Gorski SA,Hahn YS,Braciale TJ

14-7052 was used in in vivo experiments to reveal that c-kit(+) ILC2 interaction with IL-33 producing NKT and AM causes the accumulation of eosinophils during influenza recovery.

Sun Sep 01 00:00:00 EDT 2013

Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.

Journal of the American Chemical Society

Luchansky MS,Bailey RC

Published figure using IL-5 monoclonal antibody (Product # 14-7052-81) in Radioimmune assays

Wed Dec 21 00:00:00 EST 2011

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

14-7052 was used in an ELISA assay to suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.

Wed Jul 01 00:00:00 EDT 2009

The essential role of single Ig IL-1 receptor-related molecule/Toll IL-1R8 in regulation of Th2 immune response.

Journal of immunology (Baltimore, Md. : 1950)

Bulek K,Swaidani S,Qin J,Lu Y,Gulen MF,Herjan T,Min B,Kastelein RA,Aronica M,Kosz-Vnenchak M,Li X

14-7052 was used in an ELISpot assay to investigate the role that SIGIRR/TIR8 plays in the regulation of Th2 immune responses.

Sun Mar 01 00:00:00 EST 2009

Differential role of gamma interferon in inhibiting pulmonary eosinophilia and exacerbating systemic disease in fusion protein-immunized mice undergoing challenge infection with respiratory syncytial virus.

Journal of virology

Castilow EM,Olson MR,Meyerholz DK,Varga SM

14-7052 was used in an ELISpot assay to compare the immune response elicited by the G versus F protein of the respiratory syncytial virus, showing a differential role of gamma interferon.

Sat Mar 01 00:00:00 EST 2008

Pertussis toxin-induced cytokine differentiation and clonal expansion of T cells is mediated predominantly via costimulation.

Cellular immunology

Denkinger CM,Denkinger MD,Forsthuber TG

14-7052 was used in an ELISpot assay to discern that costimulation is the predominant mediator between pertussis toxin-induced cytokine differentiation and clonal expansion of T cells.

Thu Mar 01 00:00:00 EST 2007

Immunomodulatory effects of ultraviolet B irradiation on atopic dermatitis in NC/Nga mice.

Photodermatology, photoimmunology & photomedicine

Mutou Y,Ibuki Y,Kojima S

14-7052-81 was used in Enzyme-linked immunosorbent assay to indicate that UVB irradiation promotes the development of AD-like skin lesions in NC/Nga mice.

Wed Aug 01 00:00:00 EDT 2007

Pathogenic role for virus-specific CD4 T cells in mice with coronavirus-induced acute encephalitis.

The American journal of pathology

Anghelina D,Pewe L,Perlman S

14-7052 was used in an ELISpot assay to show that the host response to a single JHMV-specific immunodominant CD4 T-cell epitope is critical for severe disease.

Sat Jul 01 00:00:00 EDT 2006

Repeated irradiations with gamma-rays at a Dose of 0.5 Gy may exacerbate asthma.

Journal of radiation research

Fang SP,Tago F,Tanaka T,Simura N,Muto Y,Goto R,Kojima S

14-7052-81 was used in ELISA, Flow Cytometry, Functional Assay, Neutralization to examine d whether repeated 0.5 Gy gamma-rays irradiation can improve asthma in an OVA-induced asthmatic mouse model.

Wed Jun 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF

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Gene Aliases: EDF; IL-5; IL5; TRF

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UniProt ID: (Human) P05113, (Mouse) P04401

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Entrez Gene ID: (Human) 3567, (Mouse) 16191

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Function(s)

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-5 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-5 Monoclonal Antibody (TRFK5), eBioscience™

IL-5 Antibody (14-7052-81) in IS

Product Details

14-7052-81

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Radioimmune Assays (RIA)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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