Invitrogen

IL-6 Polyclonal Antibody, PeproTech®

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IL-6 Antibody (500-P56-1MG) in WB

Western Blot: To detect Murine IL-6 by Western Blot analysis IL-6 Polyclonal Antibody (Product # 500-P56-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P56-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 4 publications 4 publications

Neutralization (Neu)

View 4 publications 4 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

In vitro Assay (IV)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Immunostaining (IS)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Bacteria, Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine IL-6

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930630

Product Specific Information

AA Sequence of recombinant protein: MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-6. Anti-Murine IL-6-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-6 matrix.

Sandwich ELISA: To detect Murine IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-6 (500-P56Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-6.

Western Blot: To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.

PloS one

Nishimura K,Tanaka T,Takemura S,Tatsumi K,Wanaka A

500-P56 was used in Western Blotting to investigate the role of SNX25 expression on cytokine expression in RAW 264.7 macrophages.

Tue Oct 12 00:00:00 EDT 2021

Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine.

Communications biology

Klooster JPT,Bol-Schoenmakers M,van Summeren K,van Vliet ALW,de Haan CAM,van Kuppeveld FJM,Verkoeijen S,Pieters R

Thu May 27 00:00:00 EDT 2021

Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine.

Communications biology

Klooster JPT,Bol-Schoenmakers M,van Summeren K,van Vliet ALW,de Haan CAM,van Kuppeveld FJM,Verkoeijen S,Pieters R

Thu May 27 00:00:00 EDT 2021

A Metabolic Change towards Fermentation Drives Cancer Cachexia in Myotubes.

Biomedicines

Mannelli M,Gamberi T,Magherini F,Fiaschi T

500-P56 was used in Western Blotting to study the effects of a metabolic shift towards fermentation and induction of cachexia murine in myotubes, and show that inhibition of lactate formation impedes cachexia such that lactate dehydrogenase may act as a tool for preventing onset of cachexia.

Sun Jun 20 00:00:00 EDT 2021

Relative Contributions of B Cells and Dendritic Cells from Lupus-Prone Mice to CD4⁺ T Cell Polarization.

Journal of immunology (Baltimore, Md. : 1950)

Choi SC,Xu Z,Li W,Yang H,Roopenian DC,Morse HC,Morel L

Tue May 01 00:00:00 EDT 2018

The inner mitochondrial membrane protein ANT1 modulates IL-6 expression via the JNK pathway in macrophages.

FEBS letters

Nakahara K,Tanaka T,Okuda H,Isonishi A,Morita-Takemura S,Tatsumi K,Wanaka A

500-P56 was used in Western Blotting to focus on the relationship between inflammation and adenine nucleotide translocator type 1 (ANT1), which is localized in the mitochondrial inner membrane.

Thu Nov 01 00:00:00 EDT 2018

Modulation of IL-6 induced RANKL expression in arthritic synovium by a transcription factor SOX5.

Scientific reports

Feng X,Shi Y,Xu L,Peng Q,Wang F,Wang X,Sun W,Lu Y,Tsao BP,Zhang M,Tan W

500-P56 was used in Tissue/Organ culture to study the regulation of SOX5 levels in relation to receptor activator of nuclear factor κB ligand (RANKL) expression in rheumatoid arthritis (RA) in synovial fibroblasts (SF) and the development of bone erosion in the collagen-induced arthritis (CIA) mouse.

Tue Aug 23 00:00:00 EDT 2016

IL-6 Contributes to the Defective Osteogenesis of Bone Marrow Stromal Cells from the Vertebral Body of the Glucocorticoid-Induced Osteoporotic Mouse.

PloS one

Li X,Zhou ZY,Zhang YY,Yang HL

500-P56 was used in Neutralization experiments to study what leads to the defective bone marrow stromal cells (BMSC) osteogenesis in osteoporosis to provide a deeper insight into the pathophysiology of osteoporosis and identify IL-6 as a promising target for osteoporosis therapy.

Wed Apr 12 00:00:00 EDT 2017

Ovarian cancer progression is controlled by phenotypic changes in dendritic cells.

The Journal of experimental medicine

Scarlett UK,Rutkowski MR,Rauwerdink AM,Fields J,Escovar-Fadul X,Baird J,Cubillos-Ruiz JR,Jacobs AC,Gonzalez JL,Weaver J,Fiering S,Conejo-Garcia JR

Mon Mar 12 00:00:00 EDT 2012

Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources.

PloS one

Tayabali AF,Nguyen KC,Shwed PS,Crosthwait J,Coleman G,Seligy VL

Thu Sep 27 00:00:00 EDT 2012

Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources.

PloS one

Tayabali AF,Nguyen KC,Shwed PS,Crosthwait J,Coleman G,Seligy VL

Thu Sep 27 00:00:00 EDT 2012

Poor cerebral inflammatory response in eIF2B knock-in mice: implications for the aetiology of vanishing white matter disease.

PloS one

Cabilly Y,Barbi M,Geva M,Marom L,Chetrit D,Ehrlich M,Elroy-Stein O

Fri May 03 00:00:00 EDT 2013

TGF-beta-exposed plasmacytoid dendritic cells participate in Th17 commitment.

Journal of immunology (Baltimore, Md. : 1950)

Bonnefoy F,Couturier M,Clauzon A,Rémy-Martin JP,Gaugler B,Tiberghien P,Chen W,Saas P,Perruche S

Wed Jun 01 00:00:00 EDT 2011

Homocysteine suppresses the expression of the collagen cross-linker lysyl oxidase involving IL-6, Fli1, and epigenetic DNA methylation.

The Journal of biological chemistry

Thaler R,Agsten M,Spitzer S,Paschalis EP,Karlic H,Klaushofer K,Varga F

Fri Feb 18 00:00:00 EST 2011

Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.

BMC gastroenterology

Maldonado Galdeano C,Novotny Núñez I,de Moreno de LeBlanc A,Carmuega E,Weill R,Perdigón G

Thu May 26 00:00:00 EDT 2011

Cortistatin, a new antiinflammatory peptide with therapeutic effect on lethal endotoxemia.

The Journal of experimental medicine

Gonzalez-Rey E,Chorny A,Robledo G,Delgado M

Mon Mar 20 00:00:00 EST 2006

The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity.

Clinical and vaccine immunology : CVI

Galdeano CM,Perdigón G

Wed Feb 01 00:00:00 EST 2006

BCL-6 negatively regulates macrophage proliferation by suppressing autocrine IL-6 production.

Blood

Yu RY,Wang X,Pixley FJ,Yu JJ,Dent AL,Broxmeyer HE,Stanley ER,Ye BH

Tue Feb 15 00:00:00 EST 2005

Effects of milk fermented by Lactobacillus helveticus R389 on a murine breast cancer model.

Breast cancer research : BCR

de Moreno de LeBlanc A,Matar C,LeBlanc N,Perdigón G

Wed Jan 25 00:00:00 EST 2006

Effects of milk fermented by Lactobacillus helveticus R389 on a murine breast cancer model.

Breast cancer research : BCR

de Moreno de LeBlanc A,Matar C,LeBlanc N,Perdigón G

Wed Jan 25 00:00:00 EST 2006

STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia.

The Journal of clinical investigation

Lentsch AB,Kato A,Davis B,Wang W,Chao C,Edwards MJ

Thu Nov 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Il-6; Il6

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UniProt ID: (Mouse) P08505

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Entrez Gene ID: (Mouse) 16193

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Function(s)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-6 Polyclonal Antibody, PeproTech®

IL-6 Antibody (500-P56-1MG) in WB

Product Details

500-P56-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Neutralization (Neu)

Blocking Assay (BLOCK)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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