Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
IL-6 Antibody (500-P73-1MG) in ELISA
Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-6 Polyclonal Antibody (Product # 500-P73-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-6 Polyclonal Antibody, Biotin (Product # 500-P73BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-6.
Product Details
500-P73-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat IL-6
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930726
Product Specific Information
AA Sequence of recombinant protein: MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIL EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-6. Anti-Rat IL-6-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-6 matrix.
Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-6 (500-P73Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-6.
Western Blot: To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-IL-6; IL-6; ILN; Interleukin; Interleukin 6 (interferon, beta 2); Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: Ifnb2; Il-6; Il6; ILg6
UniProt ID: (Rat) P20607
Entrez Gene ID: (Rat) 24498
neutrophil apoptotic process
response to yeast
myeloid cell homeostasis
hepatic immune response
positive regulation of acute inflammatory response
negative regulation of protein kinase activity
acute-phase response
inflammatory response
aging
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell shape
response to heat
response to cold
response to wounding
response to mechanical stimulus
response to bacterium
regulation of vascular endothelial growth factor production
positive regulation of gene expression
response to auditory stimulus
response to organic cyclic compound
cell growth
cytokine-mediated signaling pathway
response to caffeine
endocrine pancreas development
neuron projection development
response to nutrient levels
response to peptidoglycan
response to lipopolysaccharide
positive regulation of chemokine production
positive regulation of interleukin-6 production
response to insulin
negative regulation of collagen biosynthetic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of protein import into nucleus, translocation
response to cytokine
wound healing
positive regulation of T cell proliferation
T cell activation
regulation of cell proliferation
response to drug
positive regulation of tyrosine phosphorylation of Stat3 protein
glucose homeostasis
defense response to protozoan
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to amino acid
positive regulation of MAPK cascade
negative regulation of chemokine biosynthetic process
regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of nitric oxide biosynthetic process
cell redox homeostasis
positive regulation of T-helper 2 cell differentiation
positive regulation of neuron differentiation
negative regulation of gluconeogenesis
positive regulation of translation
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
response to antibiotic
muscle cell cellular homeostasis
bone remodeling
negative regulation of hormone secretion
negative regulation of muscle organ development
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
negative regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of B cell activation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
response to glucocorticoid
response to calcium ion
response to electrical stimulus
defense response to virus
positive regulation of protein kinase B signaling
positive regulation of transmission of nerve impulse
branching involved in salivary gland morphogenesis
epithelial cell proliferation involved in salivary gland morphogenesis
glucagon secretion
interleukin-6-mediated signaling pathway
cellular response to hydrogen peroxide
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to dexamethasone stimulus
T-helper 17 cell lineage commitment
negative regulation of neuron death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support