Invitrogen

IL-6 Polyclonal Antibody

FIGURE: 1 / 1

IL-6 Antibody (PM626) in WB

Microbiology spectrum 2022 - FIG 7 Interaction with E. coli O111:B4 lipopolysaccharide and immunomodulatory activity of PN5. (A) CD analysis of the binding structure of AMPs with lipopolysaccharide (LPS); (B) effect of PN5 (2, 4, and 8 muM) on LPS-induced TNF-alpha production. The concentrations of TNF-alpha in the culture supernatants were determined by ELISA. (C) Western blot of TNF-alpha, TLR4, and beta-actin expression. LPS-pretrea... View More

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Product Details

PM626

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant mouse IL-6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223488

Product Specific Information

PM626 targets IL-6 in ELISA, and WB applications and shows reactivity with mouse samples.

The PM626 immunogen is recombinant mouse IL-6.

PM626 detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic Potential of Antimicrobial Peptide PN5 against Multidrug-Resistant E. coli and Anti-Inflammatory Activity in a Septic Mouse Model.

Microbiology spectrum

Kang DD,Park J,Park Y

Published figure using IL-6 polyclonal antibody (Product # PM626) in Western Blot

Wed Oct 26 00:00:00 EDT 2022

Metabolic and neuroprotective effects of dapagliflozin and liraglutide in diabetic mice.

The Journal of endocrinology

Millar P,Pathak N,Parthsarathy V,Bjourson AJ,O'Kane M,Pathak V,Moffett RC,Flatt PR,Gault VA

PM626 was used in ELISA, Western Blot, Immunohistochemistry to demonstrate that combination therapy of dapagliflozin and liraglutide exerts beneficial metabolic and neuroprotective effects in diet-induced diabetic mice.

Fri Sep 01 00:00:00 EDT 2017

Aldosterone-induced cardiomyocyte growth, and fibroblast migration and proliferation are mediated by TRAF3IP2.

Cellular signalling

Somanna NK,Yariswamy M,Garagliano JM,Siebenlist U,Mummidi S,Valente AJ,Chandrasekar B

PM626 was used in western blot to test if Aldo-mediated cardiomycocyte hypertrophy is dependent on TRAF3IP2.

Thu Oct 01 00:00:00 EDT 2015

Photodynamic therapy can induce a protective innate immune response against murine bacterial arthritis via neutrophil accumulation.

PloS one

Tanaka M,Mroz P,Dai T,Huang L,Morimoto Y,Kinoshita M,Yoshihara Y,Nemoto K,Shinomiya N,Seki S,Hamblin MR

PM626 was used in blocking or activating experiment to study the protective effect of photodynamic therapy against bacterial arthritis and the mechanism involved

Fri Jan 04 00:00:00 EST 2013

Rosuvastatin reduces microglia in the brain of wild type and ApoE knockout mice on a high cholesterol diet; implications for prevention of stroke and AD.

Biochemical and biophysical research communications

Famer D,Wahlund LO,Crisby M

PM626 was used in immunohistochemistry to study the effect of rosuvastatin on microglia in mice

Fri Nov 12 00:00:00 EST 2010

Adenosine A2A receptor deficiency exacerbates white matter lesions and cognitive deficits induced by chronic cerebral hypoperfusion in mice.

Journal of the neurological sciences

Duan W,Gui L,Zhou Z,Liu Y,Tian H,Chen JF,Zheng J

PM626 was used in western blot to study the effect of adenosine A2A receptor deficiency on chronic ischemic brain injury

Thu Oct 15 00:00:00 EDT 2009

Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-alpha challenge in mice.

Journal of immunology (Baltimore, Md. : 1950)

Starnes HF,Pearce M,Yim J,Abrams J,Tewari A,Zou J

PM626 was used in blocking/activating experiment to study the effect of interleukin 6 antibodies on Escherichia coli infection and tumor necrosis factor alpha challenge in mice

Sun Mar 15 00:00:00 EST 1992

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Il-6; Il6

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UniProt ID: (Mouse) P08505

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Entrez Gene ID: (Mouse) 16193

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Function(s)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-6 Polyclonal Antibody

IL-6 Antibody (PM626) in WB

Product Details

PM626

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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