Invitrogen

IL-6 Monoclonal Antibody (32C11)

1 Published Figure
5 References
View all (212) IL-6 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MM600D

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
View 4 publications 4 publications

Neutralization (Neu)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

32C11

Immunogen

Recombinant mouse IL-6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223536

Product Specific Information

MM600D targets IL-6 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM600D immunogen is recombinant mouse IL-6.

MM600D detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

The MM600D mouse IL6 antibody (clone 32C11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM600CB (biotinylated conjugate of clone 20F3). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM600D (clone 32C11) and biotinylated antibody MM600CB (clone 20F3) have successfully been used in combination with recombinant mouse IL6 protein SMIL6 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Il-6; Il6

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UniProt ID: (Mouse) P08505

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Entrez Gene ID: (Mouse) 16193

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Function(s)
receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity
Process(es)
neutrophil apoptotic process myeloid cell homeostasis hepatic immune response positive regulation of acute inflammatory response negative regulation of protein kinase activity acute-phase response inflammatory response immune response positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to wounding regulation of vascular endothelial growth factor production positive regulation of gene expression cell growth cytokine-mediated signaling pathway endocrine pancreas development neuron projection development positive regulation of chemokine production positive regulation of interleukin-6 production negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of protein import into nucleus, translocation positive regulation of T cell proliferation T cell activation regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis defense response to protozoan regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade negative regulation of chemokine biosynthetic process regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade muscle cell cellular homeostasis negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation negative regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation positive regulation of B cell activation positive regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity response to glucocorticoid defense response to virus positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor T-helper 17 cell lineage commitment negative regulation of neuron death
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