Invitrogen

IL-6 Recombinant Rabbit Monoclonal Antibody (4H16L21)

1 Published Figure
2 References
View all (212) IL-6 antibodies
Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

701028

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-1 µg/mL
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Immunocytochemistry (ICC/IF)

1:200
-

Flow Cytometry (Flow)

1:100
-

ELISA (ELISA)

Assay-dependent
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

4H16L21

Immunogen

proprietary

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532352

Product Specific Information

701028 was successfully to detect IL-6 in Flow application.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231, (Mouse) P08505

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Entrez Gene ID: (Human) 3569, (Mouse) 16193

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Function(s)
cytokine activity interleukin-6 receptor binding protein binding growth factor activity receptor binding
Process(es)
neutrophil apoptotic process myeloid cell homeostasis hepatic immune response positive regulation of acute inflammatory response negative regulation of protein kinase activity acute-phase response inflammatory response immune response positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to wounding regulation of vascular endothelial growth factor production positive regulation of gene expression cell growth cytokine-mediated signaling pathway endocrine pancreas development neuron projection development positive regulation of chemokine production positive regulation of interleukin-6 production negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of protein import into nucleus, translocation positive regulation of T cell proliferation T cell activation regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis defense response to protozoan regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade negative regulation of chemokine biosynthetic process regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade muscle cell cellular homeostasis negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation negative regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation positive regulation of B cell activation positive regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity response to glucocorticoid defense response to virus positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor T-helper 17 cell lineage commitment negative regulation of neuron death response to yeast neutrophil mediated immunity monocyte chemotaxis positive regulation of leukocyte chemotaxis humoral immune response aging response to heat response to cold negative regulation of lipid storage response to auditory stimulus platelet activation response to caffeine response to nutrient levels response to peptidoglycan response to insulin cellular response to hepatocyte growth factor stimulus response to drug response to amino acid negative regulation of fat cell differentiation positive regulation of osteoblast differentiation regulation of angiogenesis response to antibiotic bone remodeling defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity response to calcium ion response to electrical stimulus cellular response to dexamethasone stimulus positive regulation of cell proliferation in bone marrow positive regulation of STAT protein import into nucleus positive regulation of type B pancreatic cell apoptotic process
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